Chernova O A, Medvedeva E S, Mouzykantov A A, Baranova N B, Chernov V M
Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Lobachevskogo Str., 2/31, 420111, Kazan, Russia ; Kazan (Volga Region) Federal University, Kremlevskaya Str., 18, 420008, Kazan, Russia.
Acta Naturae. 2016 Apr-Jun;8(2):24-34.
The present review discusses the problem of controlling mycoplasmas (class Mollicutes), the smallest of self-replicating prokaryotes, parasites of higher eukaryotes, and main contaminants of cell cultures and vaccines. Possible mechanisms for the rapid development of resistance to antimicrobial drugs in mycoplasmas have been analyzed. Omics technologies provide new opportunities for investigating the molecular basis of bacterial adaptation to stress factors and identifying resistomes, the total of all genes and their products contributing to antibiotic resistance in microbes. The data obtained using an integrated approach with post-genomics methods show that antibiotic resistance may be caused by more complex processes than has been believed heretofore. The development of antibiotic resistance in mycoplasmas is associated with essential changes in the genome, proteome, and secretome profiles, which involve many genes and proteins related to fundamental cellular processes and virulence.
本综述讨论了控制支原体（柔膜菌纲）的问题，支原体是最小的自我复制原核生物，是高等真核生物的寄生虫，也是细胞培养物和疫苗的主要污染物。分析了支原体对抗菌药物快速产生耐药性的可能机制。组学技术为研究细菌适应应激因素的分子基础和鉴定耐药基因组提供了新机会，耐药基因组是所有有助于微生物产生抗生素耐药性的基因及其产物的总和。使用后基因组学方法的综合方法获得的数据表明，抗生素耐药性可能是由比以往认为的更为复杂的过程引起的。支原体抗生素耐药性的发展与基因组、蛋白质组和分泌蛋白质组谱的本质变化有关，这些变化涉及许多与基本细胞过程和毒力相关的基因和蛋白质。
