利用基因组浅层测序技术开发作物野生近缘种的微卫星标记
Microsatellite marker development in the crop wild relative using genome skimming.
作者信息
Landoni Beatrice, Viruel Juan, Gómez Rocio, Allaby Robin G, Brennan Adrian C, Picó F Xavier, Pérez-Barrales Rocio
机构信息
School of Biological Sciences University of Portsmouth PO1 2DY Portsmouth United Kingdom.
Royal Botanic Gardens, Kew TW9 3AE Richmond, London United Kingdom.
出版信息
Appl Plant Sci. 2020 May 26;8(5):e11349. doi: 10.1002/aps3.11349. eCollection 2020 May.
PREMISE
Nuclear microsatellite markers were developed for , the sister species of the crop , to provide molecular genetic tools for the investigation of genetic diversity and structure.
METHODS AND RESULTS
Fifty microsatellite loci were identified in by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the reference nuclear genome were used for genotyping six populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectively. All 16 loci successfully cross-amplified in .
CONCLUSIONS
The 16 microsatellite loci developed here can be used for population genetic studies in , and 28 additional loci that successfully amplified are available for further testing.
前提
为该作物的近缘物种开发了核微卫星标记，以提供用于研究遗传多样性和结构的分子遗传工具。
方法与结果
通过基因组扫描在该物种中鉴定出50个微卫星位点，其中44个位点成功扩增。在这些位点中，16个均匀分布在该物种参考核基因组上的位点用于对6个该物种群体进行基因分型。排除一个单态位点后，每个位点的等位基因数范围为2至12个。六个群体中有四个群体含有私有等位基因。预期杂合度和观察到的杂合度水平分别为0.076至0.667和0.000至1.000。所有16个位点在另一物种中均成功进行了交叉扩增。
结论
此处开发的16个微卫星位点可用于该物种的群体遗传学研究，另外成功扩增的28个位点可用于进一步测试。
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