Infection Control Center, Xiangya Hospital, Central South University, Changsha, China.
Changsha Hospital of Traditional Chinese Medicine, Changsha, China.
Front Cell Infect Microbiol. 2020 May 11;10:190. doi: 10.3389/fcimb.2020.00190. eCollection 2020.
The gut microbiota composition of intensive care unit (ICU) patients suffering from -positive diarrhea (CDpD) is poorly understood. This prospective study aims to use 16S rDNA (and metagenome) sequencing to compare the microbiota composition of 58 (and 5) ICU patients with CDpD (CDpD group), 33 (and 4) ICU patients with -negative diarrhea (CDnD group), and 21 (and 5) healthy control subjects (control group), as well as CDpD patients in the AB ( = 34; A/B: ), AB ( = 7), and AB ( = 17) subgroups. For 16S rDNA data, OTU clustering (tool: UPARSE), taxonomic assignment (tool: RDP classifier), α-diversity, and β-diversity analyses (tool: QIIME) were conducted. For metagenome data, metagenome assembly (tool: SOAPdenovo), gene calling (tools: MetaGeneMark, CD-HIT, and SoapAligner), unigene alignment (tool: DIAMOND), taxon difference analysis (tool: Metastats), and gene annotation (tool: DIAMOND) were performed. The microbial diversity of the CDpD group was lower than that of the CDnD and control groups. The abundances of 10 taxa (e.g., Deferribacteres, Cryptomycota, Acetothermia) were significantly higher in the CDpD group than in the CDnD group. The abundances of Saccharomycetes and Clostridia were significantly lower in CDpD in comparison with control. Some taxa were significantly different between the AB and AB subgroups. CDpD might relate to a decrease in beneficial taxa (i.e., Saccharomycetes and Clostridia) and an increase in harmful taxa (e.g., Deferribacteres, Cryptomycota, Acetothermia) in gut microbiota of ICU patients. toxin type might be slightly associated with gut microbiota composition.
重症监护病房（ICU）中发生+阳性腹泻（CDpD）的患者的肠道微生物群落组成尚不清楚。本前瞻性研究旨在使用 16S rDNA（和宏基因组）测序来比较 58 例（和 5 例）CDpD 患者（CDpD 组）、33 例（和 4 例）-阴性腹泻（CDnD 组）的 ICU 患者以及 21 例（和 5 例）健康对照者（对照组）以及 AB（=34；A/B：）、AB（=7）和 AB（=17）亚组的 CDpD 患者的微生物群落组成。对于 16S rDNA 数据，进行了 OTU 聚类（工具：UPARSE）、分类分配（工具：RDP 分类器）、α多样性和β多样性分析（工具：QIIME）。对于宏基因组数据，进行了宏基因组组装（工具：SOAPdenovo）、基因调用（工具：MetaGeneMark、CD-HIT 和 SoapAligner）、unigene 比对（工具：Diamond）、分类群差异分析（工具：Metastats）和基因注释（工具：Diamond）。CDpD 组的微生物多样性低于 CDnD 组和对照组。Deferribacteres、Cryptomycota、Acetothermia 等 10 种分类群的丰度在 CDpD 组中明显高于 CDnD 组。与对照相比，Saccharomycetes 和 Clostridia 的丰度在 CDpD 中明显降低。AB 和 AB 亚组之间的一些分类群存在显著差异。CDpD 可能与 ICU 患者肠道微生物群落中有益分类群（即 Saccharomycetes 和 Clostridia）减少和有害分类群（如 Deferribacteres、Cryptomycota、Acetothermia）增加有关。毒素类型可能与肠道微生物群落组成略有相关。
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