Zhang Xiao-Tian, Yang Yue, Ji Chunlei, Fu Yujuan, Pu Xinyi, Xu Guangyu
Department of Clinical Laboratory, The Second Hospital of Jilin University, Changchun, Jilin, 130000, China.
College of Pharmacy, Beihua University, 3999 Binjiang East Road, Jilin, Jilin, 132013, China.
Heliyon. 2024 Aug 2;10(15):e35559. doi: 10.1016/j.heliyon.2024.e35559. eCollection 2024 Aug 15.
Acute liver injury (ALI) is an abnormal liver function caused by oxidative stress, inflammation and other mechanisms.The interaction between intestine and liver plays an important role in ALI, and natural polysaccharides can participate in the regulation of ALI by regulating the composition of intestinal flora. In this study, polysaccharide was used as the research object, and ICR mice were used to construct an acute liver injury model induced by carbon tetrachloride (CCl). 16S rRNA sequencing technology was used to analyze the flora structure abundance and detect the changes of intestinal flora. The effective reading of 8 samples was obtained by 16S rRNA sequencing technology, and a total of 1233 samples were obtained. The results of alpha diversity analysis showed that the sequencing depth was sufficient, the abundance of species in the samples was high and the distribution was uniform, and the sequencing data of the samples was reasonable. Nine species with significant differences were screened out by abundence analysis of intestinal flora structure at genus level. Beta diversity analysis showed that species composition was different between the model group and the treatment group. polysaccharide can maintain the integrity of mucosal barrier by promoting the proliferation of intestinal epithelial cells and anti-oxidative stress injury, thereby improving the intestinal mucosal inflammation of mice, regulating intestinal flora, and effectively alleviating CCl-induced acute liver injury.
急性肝损伤（ALI）是由氧化应激、炎症等机制引起的肝功能异常。肠肝相互作用在ALI中起重要作用，天然多糖可通过调节肠道菌群组成参与ALI的调控。本研究以多糖为研究对象，采用ICR小鼠构建四氯化碳（CCl）诱导的急性肝损伤模型。运用16S rRNA测序技术分析菌群结构丰度，检测肠道菌群变化。通过16S rRNA测序技术获得8个样本的有效读数，共获得1233个样本。α多样性分析结果表明测序深度足够，样本中物种丰度高且分布均匀，样本测序数据合理。通过属水平肠道菌群结构丰度分析筛选出9个有显著差异的物种。β多样性分析表明模型组和治疗组之间物种组成不同。多糖可通过促进肠上皮细胞增殖和抗氧化应激损伤来维持黏膜屏障的完整性，从而改善小鼠肠道黏膜炎症，调节肠道菌群，有效减轻CCl诱导的急性肝损伤。
