Alzahrani Ashwag J, Al-Hebshi Basma M, Yahia Zolfekar A, Al-Judaibi Effat A, Alsaadi Khloud H, Al-Judaibi Awatif A
Department of Biological Sciences, Microbiology Section, College of Science, University of Jeddah, Jeddah 21959, Saudi Arabia.
Department of Internal Medicine, Al Noor Specialist Hospital, Ministry of Health, Makkah 24242, Saudi Arabia.
Microorganisms. 2025 Mar 21;13(4):710. doi: 10.3390/microorganisms13040710.
Inflammatory bowel disease (IBD) is a chronic condition that includes two main types, Crohn's disease (CD) and ulcerative colitis (UC), involving inflammation of the gastrointestinal (GI) tract. The exact cause of IBD is unknown but could be a combination of genetic, environmental, and immune system factors. This study investigated the impact of IBD on microbiota diversity by evaluating the differences in microbial composition and the microbiota of a control group (A) of healthy individuals and a group (B) of IBD patients. Sixty biopsies were collected from participants recruited from hospitals in Makkah, Saudi Arabia. Biopsy specimens were taken during colonoscopy examination, and bacterial identification was performed by extracting ribosomal DNA from sigmoid colon biopsies using a DNeasy Blood & Tissue Kit. Metagenomics and bioinformatics analyses were then conducted to analyze and compare the microbiota in the two groups. The results showed that the varieties of core microbiome species were 3.81% greater in the IBD patients than in the members of the control group. Furthermore, the differences between the groups were significantly greater than the variations within each group. Differences between the two groups were detected in the relative abundance of , , , and . These results indicate that microbiota could play a role in the pathogenesis of IBD and suggest that microbial diversity can serve as a biomarker for diagnosing the disease and monitoring its progression.
炎症性肠病（IBD）是一种慢性病，包括两种主要类型，即克罗恩病（CD）和溃疡性结肠炎（UC），涉及胃肠道（GI）的炎症。IBD的确切病因尚不清楚，但可能是遗传、环境和免疫系统因素共同作用的结果。本研究通过评估健康个体对照组（A）和IBD患者组（B）的微生物组成差异和微生物群，调查了IBD对微生物群多样性的影响。从沙特阿拉伯麦加的医院招募的参与者中收集了60份活检样本。活检标本在结肠镜检查期间采集，通过使用DNeasy Blood & Tissue试剂盒从乙状结肠活检样本中提取核糖体DNA进行细菌鉴定。然后进行宏基因组学和生物信息学分析，以分析和比较两组中的微生物群。结果显示，IBD患者的核心微生物物种种类比对照组成员多3.81%。此外，两组之间的差异明显大于每组内部的变异。在 、 、 和 的相对丰度方面检测到两组之间存在差异。这些结果表明，微生物群可能在IBD的发病机制中起作用，并表明微生物多样性可作为诊断该疾病和监测其进展的生物标志物。
