Jiang Fuchun, Zhang He-Nan, Zhang Lei, Feng Jie, Wang Wen-Han, Zhang Zhong, Musa Abubakr, Wu Di, Yang Yan
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, No. 1000 Jinqi Road, Shanghai 201403, China.
National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R. China.
Int J Med Mushrooms. 2020;22(2):183-195. doi: 10.1615/IntJMedMushrooms.2020033699.
The objective of this study was to explore the effect of magnesium acetate (MA) addition on the endo-polyphenol yield by Phellinus baumii and establish a feasible additive strategy. The optimal three-point MA addition strategy (0.05 g/L concentration of MA added at 0 h and 6 h, 0.9 g/L concentration of MA added at 12 h) was employed to obtain maximum endo-polyphenol yield. The maximum endo-polyphenol production was reached at 1.22 g/L, which was 1.39-fold higher than that of the control. Additionally, the endo-polyphenol showed stronger antioxidant activity in vitro compared with the control, including DPPH· scavenging capacity (78.76%) and Trolox equivalent antioxidant capacity (TEAC) (32.28 μmol Trolox/g sample). HPLC analysis showed that the endo-polyphenol production of the crude ethanol extracts was significantly higher than that of the control. Hispidin was isolated and identified from the ethanol extract of the culture mycelia from Ph. baumii with the three-point MA addition strategy. Hispidin showed a strong ability to scavenge DPPH free radicals and TEAC, equivalent to positive (vitamin C) value of 89.41% and 75.98%, respectively. Furthermore, hispidin protected H2O2-induced PC12 cells injured by decreased oxidative stress level. These results indicated that the MA multi-stage addition strategy was dependable, and could be used to develop new natural antioxidants for foods or medicines.
本研究的目的是探讨添加乙酸镁（MA）对鲍氏层孔菌胞内多酚产量的影响，并建立一种可行的添加策略。采用最佳的三点MA添加策略（在0小时和6小时添加浓度为0.05 g/L的MA，在12小时添加浓度为0.9 g/L的MA）以获得最大的胞内多酚产量。最大胞内多酚产量达到1.22 g/L，比对照高1.39倍。此外，与对照相比，胞内多酚在体外表现出更强的抗氧化活性，包括DPPH·清除能力（78.76%）和Trolox等效抗氧化能力（TEAC）（32.28 μmol Trolox/g样品）。HPLC分析表明，粗乙醇提取物的胞内多酚产量显著高于对照。采用三点MA添加策略从鲍氏层孔菌培养菌丝体的乙醇提取物中分离并鉴定出了苔色酸。苔色酸表现出很强的清除DPPH自由基和TEAC的能力，分别相当于阳性对照（维生素C）值的89.41%和75.98%。此外，苔色酸通过降低氧化应激水平保护H2O2诱导的PC12细胞损伤。这些结果表明，MA多阶段添加策略是可靠的，可用于开发新的食品或药物天然抗氧化剂。
