Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
Biosens Bioelectron. 2020 Sep 15;164:112309. doi: 10.1016/j.bios.2020.112309. Epub 2020 May 21.
Excessive production of uric acid (UA) in blood may lead to gout, hyperuricaemia and kidney disorder; thus, a fast, simple and reliable biosensor is needed to routinely determine the UA concentration in blood without pretreatment. The purpose of this study was to develop a mobile healthcare (mHealth) system using a drop of blood, which comprised a lateral flow pad (LFP), mesoporous Prussian blue nanoparticles (MPBs) as artificial nanozymes and auto-calculation software for on-site determination of UA in blood and data management. A standard curve was found to be linear in the range of 1.5-8.5 mg/dL UA, and convenience, cloud computing and personal information management were simultaneously achieved for the proposed mHealth system. Our mHealth system appropriately met the requirements of application in patients' homes, with the potential of real-time monitoring by their primary care physicians (PCPs).
血液中尿酸（UA）的过量产生可能导致痛风、高尿酸血症和肾脏疾病；因此，需要一种快速、简单和可靠的生物传感器来常规地测定血液中的 UA 浓度，而无需预处理。本研究的目的是开发一种使用一滴血液的移动医疗（mHealth）系统，该系统包括一个横向流动垫（LFP）、作为人工纳米酶的中孔普鲁士蓝纳米粒子（MPBs）以及用于现场测定血液中 UA 和数据管理的自动计算软件。发现标准曲线在 1.5-8.5mg/dL UA 的范围内呈线性，同时为所提出的 mHealth 系统实现了便利性、云计算和个人信息管理。我们的 mHealth 系统适当地满足了在患者家中应用的要求，具有通过他们的初级保健医生（PCP）进行实时监测的潜力。
