School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
Department of Biomedical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
Biosensors (Basel). 2020 Nov 21;10(11):187. doi: 10.3390/bios10110187.
Paper-based microfluidic analysis devices (μPADs) have attracted attention as a cost-effective platform for point-of-care testing (POCT), food safety, and environmental monitoring. Recently, three-dimensional (3D)-μPADs have been developed to improve the performance of μPADs. For accurate diagnosis of diseases, however, 3D-μPADs need to be developed to simultaneously detect multiple biomarkers. Here, we report a 3D-μPADs platform for the detection of multiple biomarkers that can be analyzed and diagnosed with a smartphone. The 3D-μPADs were fabricated using a 3D digital light processing printer and consisted of a sample reservoir (300 µL) connected to 24 detection zones (of 4 mm in diameter) through eight microchannels (of 2 mm in width). With the smartphone application, eight different biomarkers related to various diseases were detectable in concentrations ranging from normal to abnormal conditions: glucose (0-20 mmol/L), cholesterol (0-10 mmol/L), albumin (0-7 g/dL), alkaline phosphatase (0-800 U/L), creatinine (0-500 µmol/L), aspartate aminotransferase (0-800 U/L), alanine aminotransferase (0-1000 U/L), and urea nitrogen (0-7.2 mmol/L). These results suggest that 3D-µPADs can be used as a POCT platform for simultaneous detection of multiple biomarkers.
基于纸张的微流控分析器件（μPAD）作为一种具有成本效益的即时检测（POCT）、食品安全和环境监测平台，受到了广泛关注。最近，三维（3D）-μPAD 已被开发出来，以提高 μPAD 的性能。然而，为了进行准确的疾病诊断，3D-μPAD 需要开发成能够同时检测多种生物标志物。在这里，我们报告了一种用于检测多种生物标志物的 3D-μPAD 平台，该平台可通过智能手机进行分析和诊断。3D-μPAD 是使用 3D 数字光处理打印机制造的，由一个 300 µL 的样品库通过八个微通道（宽 2 毫米）连接到 24 个检测区（直径 4 毫米）组成。通过智能手机应用程序，可以检测到与各种疾病相关的八种不同的生物标志物，其浓度范围从正常到异常：葡萄糖（0-20mmol/L）、胆固醇（0-10mmol/L）、白蛋白（0-7g/dL）、碱性磷酸酶（0-800U/L）、肌酐（0-500µmol/L）、天冬氨酸氨基转移酶（0-800U/L）、丙氨酸氨基转移酶（0-1000U/L）和尿素氮（0-7.2mmol/L）。这些结果表明，3D-μPAD 可用作同时检测多种生物标志物的 POCT 平台。
