Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization, Kumamoto, Japan.
Agricultural Research Center, National Agriculture and Food Research Organization, Ibaraki, Japan.
PLoS One. 2020 Jun 1;15(6):e0233722. doi: 10.1371/journal.pone.0233722. eCollection 2020.
Melon yellow spot orthotospovirus (MYSV), a member of the genus Orthotospovirus, is an important virus in cucurbits. Thrips palmi is considered the most serious pest of cucurbits because it directly damages and indirectly transmits MYSV to the plant. The effects of MYSV-infected plants on the development time, fecundity, and preference of the thrips were analyzed in this study. Our results showed that the development time of male and female thrips did not differ significantly between MYSV-infected and non-infected cucumbers. The survival rate of thrips in non-infected and MYSV-infected cucumbers were not significantly different. In a non-choice assay, T. palmi adults were released on non-infected and MYSV-infected cucumbers and allowed to lay eggs. The number of hatched larvae did not significantly differ between non-infected and MYSV-infected cucumbers. In a choice assay, MYSV had no detectable effect on the number of adult thrips and preceding hatched larvae. In a pull assay, the settling rate of thrips on the released plant did not differ significantly when the adult thrips were released to non-infected or MYSV infected cucumbers for any cucumber cultivar. Based on our results, we propose that the effects of MYSV-infected cucumbers on the development time, fecundity, or preference of T. palmi may not be an important factor in MYSV spread between cucumbers.
瓜类黄斑坏死病毒（MYSV）是正呼肠孤病毒属的一员，是葫芦科作物中的重要病毒。棕榈蓟马被认为是葫芦科作物最严重的害虫，因为它直接损害植物，并间接将 MYSV 传播到植物上。本研究分析了感染 MYSV 的植株对蓟马发育时间、繁殖力和偏好的影响。我们的结果表明，感染和未感染 MYSV 的黄瓜中，雌雄蓟马的发育时间没有显著差异。感染和未感染 MYSV 的黄瓜中，蓟马的存活率没有显著差异。在非选择试验中，将成年棕榈蓟马释放到未感染和感染 MYSV 的黄瓜上，并允许它们产卵。未感染和感染 MYSV 的黄瓜上孵化的幼虫数量没有显著差异。在选择试验中，MYSV 对成虫蓟马和先前孵化的幼虫数量没有明显影响。在拉力试验中，当将成年蓟马释放到未感染或感染 MYSV 的黄瓜上时，对于任何黄瓜品种，释放植物上蓟马的定殖率没有显著差异。根据我们的结果，我们提出感染 MYSV 的黄瓜对 T. palmi 的发育时间、繁殖力或偏好的影响可能不是 MYSV 在黄瓜之间传播的一个重要因素。
