Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon, Republic of Korea.
Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Republic of Korea.
Int Forum Allergy Rhinol. 2020 Jul;10(7):884-892. doi: 10.1002/alr.22568. Epub 2020 Jun 1.
Povidone-iodine (PVP-I) is well known as an antiseptic and exhibits extensive activity against various pathogens. However, due to its uniquely unpleasant nature, it cannot be used locally to deactivate various sinonasal pathogens. Therefore, we developed a PVP-I composite that blocks the unpleasant odor of PVP-I for use as a local antiseptic in the sinonasal cavity and evaluated its effect on bacterial biofilm's formation and elimination in in vivo and in vitro models.
MTT, lactate dehydrogenase, and live/dead staining assay were performed to examine the cellular toxicity of PVP-I composites on the primary human nasal epithelial and RPMI 2650 cells. Crystal violet assay was performed to quantify bacterial biofilm after treating with various agents, including PVP-I and antibiotics. Hematoxylin-and-eosin staining, live/dead staining assay, and scanning electron microscopy were conducted to evaluate the effect of PVP-I on biofilm formation in a mice biofilm model.
It was observed that the PVP-I composite did not have any significant toxic effect on the nasal epithelial cells. Furthermore, the PVP-I composite effectively inhibited the formation of bacterial biomass within a dose-dependent manner after 48 hours of incubation with Pseudomonas aeruginosa and Staphylococcus aureus. In mice, it effectively eliminated biofilm from the mucosa of the nasal cavity and maxillary sinus at the tested concentrations.
The results of this study indicate that the PVP-I composite is a promising compound that could be used locally to prevent the formation of biofilms and to eliminate them from the sinonasal cavity.
聚维酮碘（PVP-I）作为一种消毒剂广为人知，对各种病原体具有广泛的活性。然而，由于其独特的不愉快性质，不能将其局部用于使各种鼻-鼻窦病原体失活。因此，我们开发了一种 PVP-I 复合材料，可阻断 PVP-I 的不愉快气味，用作鼻-鼻窦腔的局部消毒剂，并在体内和体外模型中评估其对细菌生物膜形成和消除的影响。
通过 MTT、乳酸脱氢酶和活/死染色测定法，检测 PVP-I 复合材料对原代人鼻上皮细胞和 RPMI 2650 细胞的细胞毒性。采用结晶紫法检测用各种试剂（包括 PVP-I 和抗生素）处理后细菌生物膜的形成情况。进行苏木精-伊红染色、活/死染色测定和扫描电子显微镜检查，以评估 PVP-I 对小鼠生物膜模型中生物膜形成的影响。
观察到 PVP-I 复合材料对鼻上皮细胞没有任何显著的毒性作用。此外，在孵育 48 小时后，PVP-I 复合材料以剂量依赖性方式有效抑制铜绿假单胞菌和金黄色葡萄球菌生物量的形成。在小鼠中，以测试浓度有效地从鼻腔和上颌窦的黏膜中消除生物膜。
本研究结果表明，PVP-I 复合材料是一种有前途的化合物，可局部用于预防生物膜的形成并从鼻-鼻窦腔中消除。
