Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany.
Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany.
Behav Brain Res. 2020 Aug 17;392:112680. doi: 10.1016/j.bbr.2020.112680. Epub 2020 May 30.
Despite multiple experimental models of traumatic brain injury (TBI) being available, objective assessment of gait and motor function in rodents remains difficult; therefore, we studied the value of the CatWalk XT® gait analysis as an observer independent, automated method for outcome assessment in one of the most frequently used preclinical TBI model, the controlled cortical impact (CCI), in mice.
C57Bl/6 mice were subjected to either right parietal CCI or a sham procedure. Functional outcome was assessed using the CatWalk XT® (Version 10.6) as well as the hole board test at days one, three and seven after trauma induction.
CCI led to diffuse, asymmetric and bilateral disturbances of both static and dynamic parameters in the CatWalk XT® gait analysis. The CatWalk XT® detected even minimal but statistically significant impairments that could not have been detected by clinical assessments. Impairments of static parameters were most pronounced within the first three days and diminished thereafter, while dynamic parameters were impaired until seven days after CCI. Fittingly, mice explored the hole board significantly less on day three trauma induction.
The CatWalk XT® is a valid tool for objective assessment of motor function in the acute phase after controlled cortical impact TBI in mice. Similar to observations made in humans, CCI leads to impairments of both static and dynamic parameters of gait and motor function which persist throughout the first week after the injury.
尽管有多种创伤性脑损伤（TBI）的实验模型，但对啮齿动物步态和运动功能的客观评估仍然很困难；因此，我们研究了 CatWalk XT®步态分析作为一种观察者独立、自动化的方法，用于评估最常用的临床前 TBI 模型之一，即皮质控制冲击（CCI），在小鼠中的应用价值。
C57Bl/6 小鼠接受右侧顶叶 CCI 或假手术。在创伤诱导后第 1、3 和 7 天，使用 CatWalk XT®（版本 10.6）和洞板测试评估功能结果。
CCI 导致 CatWalk XT®步态分析中静态和动态参数的弥漫性、不对称和双侧障碍。CatWalk XT®检测到甚至最小但具有统计学意义的损伤，这些损伤无法通过临床评估检测到。静态参数的损伤在最初的三天内最为明显，此后减轻，而动态参数在 CCI 后七天内受损。恰当地说，小鼠在创伤诱导后的第三天对洞板的探索明显减少。
CatWalk XT®是一种用于客观评估小鼠皮质控制冲击性 TBI 后急性期运动功能的有效工具。与在人类中观察到的情况类似，CCI 导致步态和运动功能的静态和动态参数受损，这些损伤在损伤后第一周内持续存在。
