CAS Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, PR China; Open Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology, No. 1 Wenhai Road, Qingdao 266237, PR China; Center for Ocean Mega-Science, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, PR China.
College of Resources and Environment, Hunan Agricultural University, Changsha 410128, PR China.
Sci Total Environ. 2020 Sep 20;736:139599. doi: 10.1016/j.scitotenv.2020.139599. Epub 2020 May 22.
Ivermectin is now being used as a substitute for toxic organic biocide in marine antifouling coatings due to its environmentally friendly nature and the efficacy against parasites. However, the release performance of ivermectin from the hydrolyzed acrylic-based resin into the seawater is not clear. Moreover, the efficiency and mechanism of ivermectin in preventing biofilm or slime formation have not been fully investigated. In this study, a coating was developed by mixing ivermectin with an acrylic-based resin, silyl acrylate copolymer, and a 45-day in situ antifouling test was conducted in the Yellow Sea. Direct observation and confocal microscope investigation indicated that the polymer coating with ivermectin was effective against biofilm formation. High-throughput sequencing analysis showed that ivermectin can selectively inhibit the adhesion of microorganisms. Abundances of Gammaproteobacteria and Alphaproteobacteria decreased significantly with the increased concentration of ivermectin. As for the eukaryote community, species of Stolidobranchia and unidentified_Bacillariophyceae were proved to be sensitive to ivermectin. Therefore, the ivermectin/silyl acrylate copolymer coating is a promising substitute for marine antifouling material.
伊维菌素由于其环保性质和对寄生虫的功效，现在正被用作海洋防污涂料中有毒有机杀生剂的替代品。然而，伊维菌素从水解丙烯酸基树脂向海水中的释放性能尚不清楚。此外，伊维菌素在防止生物膜或粘液形成方面的效率和机制尚未得到充分研究。在这项研究中，通过将伊维菌素与丙烯酸基树脂、硅丙酯共聚物混合，开发了一种涂层，并在黄海进行了为期 45 天的原位防污测试。直接观察和共聚焦显微镜研究表明，含有伊维菌素的聚合物涂层能有效抑制生物膜的形成。高通量测序分析表明，伊维菌素可以选择性地抑制微生物的附着。随着伊维菌素浓度的增加，γ变形菌纲和α变形菌纲的丰度显著降低。对于真核生物群落，Stolidobranchia 和未鉴定的_Bacillariophyceae 物种被证明对伊维菌素敏感。因此，伊维菌素/硅丙酯共聚物涂层是一种有前途的海洋防污材料替代品。
