神经纤毛蛋白-2 在喉鳞状细胞癌中的表达的临床意义。

Clinical significance of neuropilin-2 expression in laryngeal squamous cell carcinoma.

机构信息

Department of Otolaryngology-Head and Neck Surgery, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, People's Republic of China.

出版信息

Am J Otolaryngol. 2020 Jul-Aug;41(4):102540. doi: 10.1016/j.amjoto.2020.102540. Epub 2020 May 7.

DOI:10.1016/j.amjoto.2020.102540

PMID:32480134

Abstract

PURPOSE

The purpose of the study is to evaluate the expression of NRP-2 and explore its role in Laryngeal squamous cell carcinoma (LSCC).

MATERIALS AND METHODS

NRP-2 expression in 70 primary LSCC tissue specimens were analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients´ survival rate. Additionally, 9 paired LSCC tissues were evaluated for NRP-2 expression by Western blotting.

RESULTS

The Western blotting indicated that NRP-2 expression levels in LSCC were significantly higher than those in the paraneoplastic tissues (P < 0.05). Immunohistochemistry staining revealed that NRP-2 was detected in all primary tumor samples, moreover, high expression of NRP-2 was significantly correlated with TNM stage (P < 0.05), clinical stage (P < 0.05), histological classification (P < 0.05), lymph node metastasis (P < 0.05) and recurrence (P = 0.001). Survival curves determined by the Kaplan-Meier method showed that high expression of NRP-2 can reduce overall survival (both group P < 0.05). Then we combined the NRP-2 expression and lymph node status, and Kaplan-Meier survival showed patients with high expression of NRP-2 or lymph node metastasis (+) had both shorter disease-free and overall survival than others (both P < 0.05). Multivariate Cox proportional hazards model analysis confirmed that histological grade (P = 0.045), lymph node metastasis (P = 0.020) and high expression of NRP-2 (P = 0.033) were statistically significant, independent predictor of prognosis.

CONCLUSIONS

NRP-2 may contribute to LSCC progression and represents as a novel prognostic indicator as well as a potential therapeutic target for LSCC.

摘要

目的

本研究旨在评估 NRP-2 的表达，并探讨其在喉鳞状细胞癌（LSCC）中的作用。

材料和方法

采用免疫组织化学法分析 70 例原发性 LSCC 组织标本中 NRP-2 的表达，并与临床病理参数和患者生存率相关。此外，通过 Western blot 法评估 9 对 LSCC 组织中 NRP-2 的表达。

结果

Western blot 结果表明，LSCC 中 NRP-2 的表达水平明显高于癌旁组织（P<0.05）。免疫组织化学染色显示，NRP-2 在所有原发性肿瘤标本中均有表达，且 NRP-2 高表达与 TNM 分期（P<0.05）、临床分期（P<0.05）、组织学分级（P<0.05）、淋巴结转移（P<0.05）和复发（P=0.001）显著相关。Kaplan-Meier 生存曲线分析显示，NRP-2 高表达可降低总生存率（两组 P<0.05）。然后我们结合 NRP-2 表达和淋巴结状态，Kaplan-Meier 生存分析显示，NRP-2 高表达或淋巴结转移阳性的患者无病生存率和总生存率均短于其他患者（均 P<0.05）。多因素 Cox 比例风险模型分析证实，组织学分级（P=0.045）、淋巴结转移（P=0.020）和 NRP-2 高表达（P=0.033）是独立的预后预测因素。