DRDO-BU Center for Life Sciences, Bharathiar University Campus, Coimbatore 641046, India; Unit of Toxicology, Department of Zoology, Bharathiar University, Coimbatore 641046, India; Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 28789 Jingshidong Road, Licheng District, Jinan 250103, China.
DRDO-BU Center for Life Sciences, Bharathiar University Campus, Coimbatore 641046, India.
Sci Total Environ. 2020 Sep 15;735:139496. doi: 10.1016/j.scitotenv.2020.139496. Epub 2020 May 17.
Pyriproxyfen (PPF), a broad-spectrum insecticide known to cause reproductive and endocrine disruption in invertebrates, while the data is scarce in aquatic vertebrates. The goal of this study is to investigate the impact of PPF on reproductive endocrine system of male and female zebrafish along hypothalamus-pituitary-gonadal (HPG) axis. In brain, PPF caused significant alteration in the transcripts of erα, lhβ, and cyp19b genes in male and fshβ, lhβ, and cyp19b genes in female zebrafish. The downstream genes of steroidogenic pathway like, star, 3βhsd, 17βhsd, and cyp19a expression were significantly altered in gonad of both sexes. Subsequent changes in circulatory steroid hormone levels lead to imbalance in hormone homeostasis as revealed from estradiol/testosterone (E/T) ratio. Further, the vitellogenin transcript level was enhanced in hepatic tissues and their blood plasma content was increased in male (16.21%) and declined in female (21.69%). PPF also induced histopathological changes in gonads such as, reduction of mature spermatocytes in male and vitellogenic oocytes in female zebrafish. The altered E/T ratio and gonadal histopathology were supported by the altered transcript levels of HPG axis genes. Overall, these findings provide new insights of PPF in zebrafish reproductive system and highlights for further investigations on its potential risks in aquatic environment.
吡丙醚（PPF）是一种广谱杀虫剂，已知会对无脊椎动物的生殖和内分泌系统造成破坏，而水生脊椎动物的数据则很少。本研究旨在探讨 PPF 对雄性和雌性斑马鱼下丘脑-垂体-性腺（HPG）轴生殖内分泌系统的影响。在大脑中，PPF 导致雄性斑马鱼中 erα、lhβ 和 cyp19b 基因以及雌性斑马鱼中 fshβ、lhβ 和 cyp19b 基因的转录物发生显著改变。类固醇生成途径的下游基因，如 star、3βhsd、17βhsd 和 cyp19a 的表达在两性性腺中均发生显著改变。随后循环类固醇激素水平的变化导致激素稳态失衡，如雌二醇/睾酮（E/T）比值所示。此外，肝组织中的卵黄蛋白原转录物水平升高，其血液中的含量在雄性（16.21%）中增加，在雌性（21.69%）中下降。PPF 还导致性腺发生组织病理学变化，如雄性成熟精母细胞减少和雌性产黄卵母细胞增多。HPG 轴基因转录物水平的改变支持了 E/T 比值和性腺组织病理学的改变。总的来说，这些发现为 PPF 在斑马鱼生殖系统中的作用提供了新的见解，并强调了进一步研究其在水生环境中潜在风险的必要性。
