Song Wei, Gan Wu, Xie Zhengli, Chen Jia, Wang Lumin
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.
Front Genet. 2022 Aug 26;13:955645. doi: 10.3389/fgene.2022.955645. eCollection 2022.
() is an economically important fish species, exhibiting sexual dimorphism in its growth rate. However, there is a lack of research on its underlying sex-related mechanisms. Therefore, small RNA sequencing was performed to better comprehend these sex-related molecular mechanisms. In total, 171 differentially expressed miRNAs (DE-miRNAs) were identified between the ovaries and testes. Functional enrichment analysis revealed that the target genes of DE-miRNAs were considerably enriched in the p53 signaling, PI3K-Akt signaling, and TGF-beta signaling pathways. In addition, sex-related miRNAs were identified, and the expression of miR-430c-3p and miR-430f-3p was specifically observed in the gonads compared with other organs and their expression was markedly upregulated in the testes relative to the ovaries. was a target of miR-430c-3p and was greatly expressed in the ovaries compared with the testes. Importantly, miR-430c-3p and co-expressed in the ovaries and testes. This research provides the first detailed miRNA profiles for concerning sex, likely laying the basis for further studies on sex differentiation in .
（某物种）是一种具有重要经济价值的鱼类，其生长速度存在性别二态性。然而，对其潜在的性别相关机制缺乏研究。因此，进行了小RNA测序以更好地理解这些性别相关的分子机制。总共在卵巢和睾丸之间鉴定出171个差异表达的miRNA（DE-miRNA）。功能富集分析表明，DE-miRNA的靶基因在p53信号通路、PI3K-Akt信号通路和TGF-β信号通路中显著富集。此外，鉴定出了与性别相关的miRNA，与其他器官相比，miR-430c-3p和miR-430f-3p在性腺中特异性表达，并且相对于卵巢，它们在睾丸中的表达明显上调。（某基因）是miR-430c-3p的靶标，与睾丸相比，在卵巢中大量表达。重要的是，miR-430c-3p和（某基因）在卵巢和睾丸中共同表达。本研究提供了关于（某物种）性别方面的首个详细miRNA图谱，可能为进一步研究（某物种）的性别分化奠定基础。
