Omar Mahmoud A, Anwer Ebtehal F, Nour El-Deen Deena A M
Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Medinah, Saudi Arabia; Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 5;239:118510. doi: 10.1016/j.saa.2020.118510. Epub 2020 May 20.
Tranexamic acid (TXA) is an important antihemorrhagic drug that needs a simple, sensitive and low cost spectrofluorimetric method for its determination. This method depends on generation of a yellow product which produced from a nucleophilic substitution reaction of the lone pair of electrons on the amino group found in the TXA chemical structure and 4-chloro-7-nitrobenzofurazan (NBD-Cl) in borate buffer PH 9.0. The product was measured at 536 nm (λex = 470.5 nm). All variables that have an effect on the formation and stability of the product have been explored and optimized. The linear range was 20-100 ng mL with a limit of quantitation 12.4 ng mL. This method has been applied for assurance of tranexamic acid in ampoules and tablets dosage forms without any interference from excipients present. Also, we study the drug in human plasma.
氨甲环酸（TXA）是一种重要的抗出血药物，需要一种简单、灵敏且低成本的荧光分光光度法来测定。该方法基于在pH 9.0的硼酸盐缓冲液中，TXA化学结构中氨基上的孤对电子与4-氯-7-硝基苯并呋喃（NBD-Cl）发生亲核取代反应生成黄色产物。产物在536 nm处测定（激发波长λex = 470.5 nm）。对影响产物形成和稳定性的所有变量进行了探索和优化。线性范围为20 - 100 ng/mL，定量限为12.4 ng/mL。该方法已用于安瓿剂和片剂剂型中氨甲环酸的测定，不受辅料干扰。此外，我们还对人血浆中的该药物进行了研究。
