Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 5;196:196-201. doi: 10.1016/j.saa.2018.02.019. Epub 2018 Feb 6.
A simple and sensitive spectrofluorimetric method has been development for the assurance of quetiapine fumarate (QTF). The proposed method was utilized for measuring the fluorescence intensity of the yellow fluorescent product at 510nm (λ 470nm). The fluorescent product has resulted from the nucleophilic substitution reaction of QTF with 4-chloro-7-nitrobenzofurazane (NBD-Cl) in Mcllvaine buffer (pH7.0). The diverse variables influencing the development of the reaction product were deliberately changed and optimized. The linear concentration range of the proposed method was of 0.2-2.0μgml.The limits of detection and quantitation were 0.05 and 0.17μgml, respectively. The proposed method was applied for the assurance of QTF in its tablets without interference from basic excipients. In addition, the proposed method was used for in vitro analysis of the QTF in spiked human plasma, the percent mean recovery was (n=3) 98.82±1.484%.
已经开发出一种简单灵敏的荧光光谱法来保证富马酸喹硫平（QTF）的含量。该方法利用 QTF 与 4-氯-7-硝基苯并呋咱（NBD-Cl）在 Mcllvaine 缓冲液（pH7.0）中发生亲核取代反应，生成黄色荧光产物，并在 510nm（λ470nm）处测量荧光强度。已经故意改变和优化了影响反应产物形成的各种变量。该方法的线性浓度范围为 0.2-2.0μgml。检测限和定量限分别为 0.05 和 0.17μgml。该方法用于测定其片剂中的 QTF，不会受到碱性赋形剂的干扰。此外，该方法还用于人血浆中 QTF 的体外分析，（n=3）平均回收率为 98.82±1.484%。
