Ji Kai, Chen Pei, Sun Liang, Wang Yanping, Dai Shengjie, Li Qian, Li Ping, Sun Yufei, Wu Yan, Duan Chaorui, Leng Ping
College of Agriculture and Biotechnology, China Agricultural University, Beijing 100 193, China.
Funct Plant Biol. 2012 May;39(4):351-357. doi: 10.1071/FP11293.
To study the role of ABA in development and ripening of strawberry fruit, two ABA key synthetase genes FaNCED1, FaNCED2 and one ABA key degradation enzyme gene FaCYP707A1 were cloned from strawberry cultivar 'Ablion'. The three genes and putative amino acid sequences, respectively, had high similarities with their homologues in other plants. In strawberry pulp, expression of FaNCED2 rose in two stages that corresponded with increases in ABA levels. The expression of FaNCED1 was much lower and increased continually. The expression of FaCYP707A1 increased as fruit changed from bright green to white, then decreased as it ripened. Auxin reduced expression of these three genes. Analysis of expression of these genes in different organs and tissues showed that FaNCED2 was abundant in mature achenes and the pulp (receptacle) had high expression of FaNCED1 and FaCYP707A1. ABA may play a regulation role in strawberry fruit development and ripening. The content of ABA was regulated by its key synthetase gene NCED2 and degradation gene CYP707A1.
为研究脱落酸（ABA）在草莓果实发育和成熟过程中的作用，从草莓品种‘阿尔比恩’中克隆了两个ABA关键合成酶基因FaNCED1、FaNCED2和一个ABA关键降解酶基因FaCYP707A1。这三个基因及其推测的氨基酸序列分别与其在其他植物中的同源物具有高度相似性。在草莓果肉中，FaNCED2的表达在两个阶段上升，这与ABA水平的增加相对应。FaNCED1的表达低得多且持续增加。FaCYP707A1的表达在果实从亮绿色变为白色时增加，然后在果实成熟时下降。生长素降低了这三个基因的表达。对这些基因在不同器官和组织中的表达分析表明，FaNCED2在成熟瘦果中含量丰富，果肉（花托）中FaNCED1和FaCYP707A1表达较高。ABA可能在草莓果实发育和成熟中起调控作用。ABA的含量受其关键合成酶基因NCED2和降解基因CYP707A1的调控。
