van der Heijden Gerie, Song Yu, Horgan Graham, Polder Gerrit, Dieleman Anja, Bink Marco, Palloix Alain, van Eeuwijk Fred, Glasbey Chris
Wageningen UR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
BioSS, King's Buildings, Edinburgh EH9 3JZ, UK.
Funct Plant Biol. 2012 Nov;39(11):870-877. doi: 10.1071/FP12019.
Most high-throughput systems for automated plant phenotyping involve a fixed recording cabinet to which plants are transported. However, important greenhouse plants like pepper are too tall to be transported. In this research we developed a system to automatically measure plant characteristics of tall pepper plants in the greenhouse. With a device equipped with multiple cameras, images of plants are recorded at a 5cm interval over a height of 3m. Two types of features are extracted: (1) features from a 3D reconstruction of the plant canopy; and (2) statistical features derived directly from RGB images. The experiment comprised 151 genotypes of a recombinant inbred population of pepper, to examine the heritability and quantitative trait loci (QTL) of the features. Features extracted from the 3D reconstruction of the canopy were leaf size and leaf angle, with heritabilities of 0.70 and 0.56 respectively. Three QTL were found for leaf size, and one for leaf angle. From the statistical features, plant height showed a good correlation (0.93) with manual measurements, and QTL were in accordance with QTL of manual measurements. For total leaf area, the heritability was 0.55, and two of the three QTL found by manual measurement were found by image analysis.
大多数用于植物自动表型分析的高通量系统都包含一个固定的记录箱，植物会被运送到该记录箱处。然而，像辣椒这样重要的温室植物太高，无法进行运输。在本研究中，我们开发了一种系统，用于自动测量温室中高大辣椒植株的植物特征。利用一个配备了多个摄像头的设备，在3米的高度上以5厘米的间隔记录植物的图像。提取了两种类型的特征：(1) 植物冠层三维重建的特征；(2) 直接从RGB图像导出的统计特征。该实验包含151个辣椒重组自交群体的基因型，以检验这些特征的遗传力和数量性状位点（QTL）。从冠层三维重建中提取的特征是叶片大小和叶角，遗传力分别为0.70和0.56。发现了三个与叶片大小相关的QTL，以及一个与叶角相关的QTL。从统计特征来看，株高与人工测量结果显示出良好的相关性（0.93），且QTL与人工测量的QTL一致。对于总叶面积，遗传力为0.55，通过人工测量发现的三个QTL中有两个也通过图像分析被发现。
