Goyal Arvind K, Basistha Bharat C, Sen Arnab, Middha Sushil K
Molecular Genetics Laboratory, Department of Botany, University of North Bengal, Siliguri 734013, India.
Department of Biotechnology, MLA College for Women, Bangalore, Karnataka, India.
Funct Plant Biol. 2011 Sep;38(9):697-701. doi: 10.1071/FP11016.
Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99±32 to 1459±53mg gallic acid equivalent g-1, 135±12 to 707±62mg quercetin equivalent g-1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1mgmL-1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200µgmL-1 with a reducing capacity of extracts as water>methanol>acetone. Strong correlation (P<0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.
沙棘俗称醋柳，是一种用途广泛的植物，具有多种用途，包括控制土壤侵蚀、生物固氮和药用特性。在印度，有两种沙棘；即柳叶沙棘（Hippophae salicifolia D. Don）和沙棘（Hippophae rhamnoides L.）非常常见。柳叶沙棘具有从灌木到乔木的习性，仅限于喜马拉雅地区，而沙棘则呈灌木状，生长在印度海拔较高的地区，并广泛分布于欧洲和亚洲。在本研究中，对柳叶沙棘雌株和雄株各部位的水提取物、甲醇提取物和丙酮提取物进行了总抗氧化活性、酚类和黄酮类含量的评估，并进行了相关性分析。所有样品中的总酚和黄酮含量分别为99±32至1459±53mg没食子酸当量g-1、135±12至707±62mg槲皮素当量g-1干提取物。在雌株树皮和雄株叶片的甲醇提取物中发现了最高的自由基（DPPH）清除活性。雄株树皮和雄株叶片的甲醇提取物在浓度为1mgmL-1时表现出最大的H2O2活性。抗氧化活性（FRAP法）在浓度为20至200µgmL-1时范围为0.021至1.041，提取物的还原能力为水>甲醇>丙酮。在所研究的所有提取物中，DPPH清除活性与总酚含量之间存在强相关性（P<0.05）。
