Lai Wenqiang, Zhuang Junyang, Que Xiaohua, Fu Libing, Tang Dianping
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, P.R. China.
Biomater Sci. 2014 Aug 30;2(8):1073-1079. doi: 10.1039/c3bm60284b. Epub 2014 Mar 10.
A new electrochemical immunosensor was designed for the determination of carcinoembryonic antigen (CEA) with sensitivity enhanced by using nanogold-poly(o-phenylenediamine)-manganese dioxide organic-inorganic hybrid nanostructures (GNPM) as nanotags and peroxidase mimics. Initially, mesoporous poly(o-phenylenediamine)-manganese dioxide (PPD-MnO) hollow microspheres were synthesized by an inorganic/organic interfacial polymerization technique. Then gold nanoparticles were assembled onto the surface of PPD-MnO, which were used for the labelling of the anti-CEA detection antibody (pAb). The prepared GNPM nanotags were characterized using transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), UV-vis absorption spectroscopy, N adsorption-desorption isotherm measurements and Fourier transform infrared spectroscopy (FTIR). The assay was carried out with a sandwich-type immunoassay format in pH 5.5 acetic acid-buffered saline solution containing 2.5 mmol L HO. Experimental results indicated that the electrochemical immunosensor exhibited a wide dynamic range from 0.01 to 80 ng mL towards the target CEA with a detection limit (LOD) of 6.0 pg mL. The immunosensor also displayed a good stability and acceptable reproducibility and selectivity. In addition, the methodology was evaluated by assaying 10 clinical serum samples, providing a good relationship between the electrochemical immunosensor and the commercialized electrochemiluminescent (ECL) method for determination of CEA.
设计了一种新型电化学免疫传感器用于检测癌胚抗原（CEA），该传感器通过使用纳米金-聚邻苯二胺-二氧化锰有机-无机杂化纳米结构（GNPM）作为纳米标签和过氧化物酶模拟物来提高灵敏度。首先，通过无机/有机界面聚合技术合成了介孔聚邻苯二胺-二氧化锰（PPD-MnO）空心微球。然后将金纳米颗粒组装到PPD-MnO表面，用于标记抗CEA检测抗体（pAb）。使用透射电子显微镜（TEM）、场发射扫描电子显微镜（FESEM）、紫外-可见吸收光谱、N吸附-脱附等温线测量和傅里叶变换红外光谱（FTIR）对制备的GNPM纳米标签进行了表征。该检测采用夹心型免疫分析形式，在含有2.5 mmol L HO的pH 5.5乙酸缓冲盐溶液中进行。实验结果表明，该电化学免疫传感器对目标CEA的动态检测范围为0.01至80 ng mL，检测限（LOD）为6.0 pg mL。该免疫传感器还表现出良好的稳定性、可接受的重现性和选择性。此外，通过检测10份临床血清样本对该方法进行了评估，结果表明电化学免疫传感器与商业化的电化学发光（ECL）法在检测CEA方面具有良好的相关性。
