Amgoth Chander, Singh Avinash, Santhosh Rompivalasa, Yumnam Sujata, Mangla Priyanka, Karthik Rajendra, Guping Tang, Banavoth Murali
Department of Science and Humanities, MLR Institute of Technology Hyderabad-500043 TS India.
School of Chemistry, University of Hyderabad Hyderabad-500046 TS India.
RSC Adv. 2019 Oct 22;9(58):33931-33940. doi: 10.1039/c9ra05484g. eCollection 2019 Oct 18.
Herein, the synthesis and characterization of ideal size (∼10 and 40 nm, in diameter) AuNPs (gold nanoparticles) were reported. Two different organic solvents such as DMF (dimethyl formamide) and NMPL (-methyl-2-pyrrolidone) were used to synthesize AuNPs along with agents reducing agents such as NaBH (sodium borohydrate) and NaCHO (sodium citrate). The combination of [(HAuCl)-(DMF)-(NaBH)] gives AuNPs with an avg. size of 10.2 nm. Similarly, the combination of [(HAuCl)-(NMPL)-(NaCHO)] gives AuNPs with an avg. size of 40.4 nm. The morphology of these nanoscale AuNPs has been characterized through TEM and HRTEM imaging followed by SAED for lattice parameters such as -spacing value (2.6 Å/0.26 nm) of crystalline metal (Au) nanoparticles. Further, these unique and ideal nanoscale AuNPs were used to evaluate the potential working efficacy by using cell based studies on K562 (leukaemia) blood cancer cells. From the MTT assay results around 88% cell inhibition was measured for ∼10 nm sized AuNPs. The treated cells were stained with different fluorescent dyes such as FITC, DAPI, Rho-6G and their ruptured morphology has been reported in the respective sections. These types of ideal sized metal (Au) nanoparticles are recommended for various theranostics such as to cure breast, colon, lung and liver cancers.
本文报道了理想尺寸（直径约10和40纳米）的金纳米颗粒（AuNPs）的合成与表征。使用两种不同的有机溶剂，如N，N-二甲基甲酰胺（DMF）和N-甲基-2-吡咯烷酮（NMPL），与硼氢化钠（NaBH）和柠檬酸钠（NaCHO）等还原剂一起合成AuNPs。[(HAuCl)-(DMF)-(NaBH)]的组合得到平均尺寸为10.2纳米的AuNPs。同样，[(HAuCl)-(NMPL)-(NaCHO)]的组合得到平均尺寸为40.4纳米的AuNPs。通过透射电子显微镜（TEM）和高分辨透射电子显微镜（HRTEM）成像以及选区电子衍射（SAED）对这些纳米级AuNPs的形态进行了表征，以确定晶体金属（Au）纳米颗粒的晶格参数，如晶面间距值（2.6埃/0.26纳米）。此外，通过对K562（白血病）血癌细胞进行基于细胞的研究，使用这些独特且理想的纳米级AuNPs来评估其潜在的工作效能。从MTT分析结果来看，约10纳米大小的AuNPs对细胞的抑制率约为88%。用不同的荧光染料如异硫氰酸荧光素（FITC）、4',6-二脒基-2-苯基吲哚（DAPI）、罗丹明-6G对处理后的细胞进行染色，并在相应部分报告了它们破裂的形态。推荐将这些理想尺寸的金属（Au）纳米颗粒用于各种治疗诊断，如治疗乳腺癌、结肠癌、肺癌和肝癌。
