Wu Xuanjun, Tian Yunpeng, Yu Mingzhu, Lin Bijuan, Han Jiahuai, Han Shoufa
Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Innovation Center for Cell Biology, Xiamen University, Xiamen, 361005, China.
Biomater Sci. 2014 Aug 30;2(8):1120-1127. doi: 10.1039/c4bm00028e. Epub 2014 May 1.
Surgical resection is widely used for tumor treatment, necessitating approaches for the precise locating of elusive tumor foci. We report the high performance detection of tumors in mice with fluorescein-isothiocyanate (FITC) labelled sialic acid (FITC-SA), a fluorescent monosaccharide with low cytoxicity. Analysis of mice intravenously injected with FITC-SA revealed high target-to-background fluorescence ratios in subcutaneous tumors and liver tumor implants with 0.2-5 mm diameters, which are significantly below the clinical threshold of minimal residual cancer (∼1 cm clearance). Extracellular FITC-SA is quickly cleared from circulation whereas the intracellular FITC-SA could be metabolically incorporated into glycoproteins via a cellular sialylation pathway. Compared with FITC-SA-laden nanoparticles, free FITC-SA is preferentially and quickly taken up by tumors in mice and displays high tumor-to-background signal contrast, suggesting the potential for fluorescence directed surgical ablation of tumors.
手术切除广泛应用于肿瘤治疗，因此需要精确找到难以捉摸的肿瘤病灶的方法。我们报告了用异硫氰酸荧光素（FITC）标记的唾液酸（FITC-SA）对小鼠肿瘤进行的高性能检测，FITC-SA是一种具有低细胞毒性的荧光单糖。对静脉注射FITC-SA的小鼠进行分析发现，在直径为0.2-5毫米的皮下肿瘤和肝肿瘤植入物中，目标与背景的荧光比率很高，这些直径明显低于最小残留癌的临床阈值（约1厘米清除率）。细胞外的FITC-SA会迅速从循环中清除，而细胞内的FITC-SA可以通过细胞唾液酸化途径代谢整合到糖蛋白中。与负载FITC-SA的纳米颗粒相比，游离的FITC-SA在小鼠体内被肿瘤优先且快速摄取，并显示出高肿瘤与背景信号对比度，这表明荧光引导肿瘤手术消融具有潜力。
