Wu Xuanjun, Lin Bijuan, Yu Mingzhu, Yang Liu, Han Jiahuai, Han Shoufa
State Key Laboratory for Physical Chemistry of Solid Surfaces , The Key Laboratory for Chemical Biology of Fujian Province , The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation , Innovation Center for Cell Biology, and Department of Chemical Biology , College of Chemistry and Chemical Engineering Xiamen University , Xiamen , 361005 , China . Email:
State Key Laboratory of Cellular Stress Biology , Innovation Center for Cell Biology , School of Life Sciences , Xiamen University , Xiamen , 361005 , China.
Chem Sci. 2015 Mar 1;6(3):2002-2009. doi: 10.1039/c4sc03641g. Epub 2014 Dec 22.
Activatable molecular systems enabling precise tumor localization are valuable for complete tumor resection. Herein, we report sialic acid-capped polymeric nanovesicles encapsulating the near infrared profluorophore (pNIR@P@SA) for lysosome activation based dual modality tumor imaging. The probe features surface-anchored sialic acid for tumor targeting and a core of near infrared profluorophore (pNIR) which undergoes lysosomal acidity triggered isomerization to give optical and optoacoustic signals upon cell internalization. Imaging studies reveal high-efficiency uptake and signal activation of pNIR@P@SA in subcutaneous tumors and millimeter-sized liver tumor foci in mice. The high tumor-to-healthy organ signal contrasts and discernment of tiny liver tumors from normal liver tissues validate the potential of pNIR@P@SA for high performance optical and optoacoustic imaging guided tumor resection.
能够实现精确肿瘤定位的可激活分子系统对于完整切除肿瘤很有价值。在此，我们报告了一种唾液酸封端的聚合物纳米囊泡，其包裹着近红外前体荧光团（pNIR@P@SA），用于基于溶酶体激活的双模态肿瘤成像。该探针具有表面锚定的唾液酸用于肿瘤靶向，以及近红外前体荧光团（pNIR）核心，其在细胞内化后会经历溶酶体酸性触发的异构化，从而产生光学和光声信号。成像研究表明，pNIR@P@SA在小鼠皮下肿瘤和毫米级肝肿瘤病灶中具有高效摄取和信号激活。高肿瘤与健康器官信号对比度以及从小鼠正常肝组织中辨别微小肝肿瘤，验证了pNIR@P@SA在高性能光学和光声成像引导肿瘤切除方面的潜力。
