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评价 KRA-28 对. 的杀线虫活性。

Evaluation of Nematicidal Activity of KRA-28 against .

机构信息

Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, Republic of Korea.

Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan 54596, Republic of Korea.

出版信息

J Microbiol Biotechnol. 2020 May 28;30(5):700-707. doi: 10.4014/jmb.1908.08038.

Abstract

The root-knot nematode () is an important pathogen in crop cultivation, however, few methods are available to control this parasitic roundworm. In this study, the nematicidal effects of approximately 30 strains isolated from soil samples of Mt. Naejang (Korea) were tested against , and the culture broth of the strains KRA- 24 and KRA-28 exhibited approximately 75% and 85% insecticidal activity, respectively, in in vitro assays. In in vivo pot experiments, these strains reduced the number of nematodes in the soil and the number of egg masses in the roots of red peppers. The two strains also survived in the presence of insecticidal agents (0.1 to 3.0%) such as fosthiazate, ethoprophos and terbufos when they were used in parallel. The mixture of KRA-24 or KRA-28 culture broth and fosthiazate exhibited nematicidal effects that were similar to those observed when KRA-24 or KRA-28 were used alone. Our results clearly suggest that the Streptomyces strains KRA-24 and KRA-28 should be promoted as a biocontrol agent against .

摘要

根结线虫()是作物栽培中的一种重要病原体,但目前可用的控制这种寄生性圆线虫的方法很少。在这项研究中,测试了大约 30 株从韩国内藏山土壤样本中分离出来的菌株对的杀线虫效果,菌株 KRA-24 和 KRA-28 的培养液在体外试验中分别表现出约 75%和 85%的杀虫活性。在体内盆栽试验中,这些菌株减少了土壤中的线虫数量和红辣椒根部的卵块数量。当这两种菌株与福噻唑、乙拌磷和涕灭威等杀虫剂(0.1 至 3.0%)同时使用时,它们也能在这些杀虫剂存在的情况下存活。KRA-24 或 KRA-28 培养液与福噻唑的混合物表现出与单独使用 KRA-24 或 KRA-28 时相似的杀线虫效果。我们的研究结果清楚地表明,应该将链霉菌菌株 KRA-24 和 KRA-28 作为防治的生物防治剂加以推广。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f68e/9728339/ed2e18645145/JMB-30-5-700-f1.jpg

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