Goodell P B, Ferris H
J Nematol. 1989 Jul;21(3):328-34.
The influence of soil temperature and moisture on Meloidogyne incognita (Kofoid and White) Chitwood was examined in relation to hatching and survival of second-stage juveniles (J2). Nematodes were cultured on cotton (Gossypium hirsutum L. cv. Acala SJ2) under field conditions to provide populations similar to those found in the field in late autumn. Egg masses were placed in a temperature range (9-12 C and 21 C), and hatch was measured over a period equivalent to 20 degree days > 10 C (DD10). Hatch occurred below the reported 18 C activity threshold, was restricted below 12 C, and was inhibited below 10 C. Soil moisture influence on hatch was measured by placing egg masses in Hesperia sandy loam and subjecting them to suction pressures ranging from -1.1 bars to -4 .5 bars. Suction potentials of less than -2 bars reduced hatch and less than -3 bars inhibited hatch. J2 were placed in sandy loam soil with soil moisture near field capacity, and their motility was measured over a period of 500 DD10. In the absence of a host, more than 90% of J2 became nonmotile over this period.
研究了土壤温度和湿度对南方根结线虫（Meloidogyne incognita (Kofoid and White) Chitwood）二龄幼虫（J2）孵化和存活的影响。在田间条件下，将线虫培养在棉花（Gossypium hirsutum L. cv. Acala SJ2）上，以获得与秋末田间发现的线虫种群相似的群体。将卵块置于一定温度范围（9 - 12℃和21℃），并在相当于20℃日（>10℃，DD10）的时间段内测量孵化情况。孵化在报道的18℃活动阈值以下发生，在12℃以下受到限制，在10℃以下受到抑制。通过将卵块放置在赫斯珀里亚砂壤土中，并使其承受从 - 1.1巴到 - 4.5巴的吸力来测量土壤湿度对孵化的影响。小于 - 2巴的吸力势降低孵化率，小于 - 3巴的吸力势抑制孵化。将J2放置在土壤湿度接近田间持水量的砂壤土中，并在500个DD10的时间段内测量其活力。在没有寄主的情况下，在此期间超过90%的J2变得无活力。
