Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia.
Siberian Federal University, 660041, Krasnoyarsk, Russia.
Dokl Biol Sci. 2020 Mar;491(1):43-46. doi: 10.1134/S0012496620020039. Epub 2020 Jun 1.
The regularities of the functioning of a number of enzymes in a viscous environment created by natural polymers, starch and gelatin are examined. Based on the analysis of kinetic curves of thermal inactivation, mechanisms of thermal inactivation of enzymes in a viscous microenvironment are proposed. Using the example of butyrylcholinesterase, NAD(P)H:FMN oxidoreductase, and coupled system of the luminous bacteria (NAD(P)H:FMN oxidoreductase + luciferase), the conditions, under which starch and gelatin have a stabilizing effect on enzyme activity during storage and exposure to various physical and chemical environmental factors, were found. A significant increase in the stabilizing effect is achieved by eliminating water during drying the enzyme preparations immobilized in starch and gelatin polymer gels.
研究了在天然聚合物（淀粉和明胶）形成的粘性环境中一些酶的作用规律。通过分析热失活动力学曲线，提出了在粘性微环境中酶热失活的机制。以丁酰胆碱酯酶、NAD(P)H：FMN 氧化还原酶和发光细菌偶联系统（NAD(P)H：FMN 氧化还原酶+荧光素酶）为例，找到了在储存和暴露于各种物理和化学环境因素时，淀粉和明胶对酶活性具有稳定作用的条件。通过在淀粉和明胶聚合物凝胶中固定化酶制剂干燥时除去水分，可以显著提高稳定效果。
