不同氧浓度下多能间充质干细胞中双向分化潜能相关基因的差异表达。
Differential Expression of Bipotent Commitment-Related Genes in Multipotent Mesenchymal Stromal Cells at Different O Levels.
机构信息
Institute of Biomedical Problems, Russian Academy of Sciences, 123007, Moscow, Russia.
出版信息
Dokl Biochem Biophys. 2020 Mar;491(1):67-69. doi: 10.1134/S1607672920020052. Epub 2020 Jun 1.
The transcriptomic profile associated with osteo- and adipogenic differentiation in growth-arrested multipotent mesenchymal stromal cells (MSCs) from human adipose tissue was analyzed in vitro at 20% (standard laboratory) and 5% (tissue-related) O levels. Compared with day 7, at 5% O on day 14 spontaneous upregulation of osteo- (RUNX2, SP7, BGLAP, and SPP1) and adipogenic differentiation (CEBPA, PPARG, and ADIPOQ) genes in MSCs was observed (p < 0.05). Thus, upon expansion under tissue-related O, MSCs demonstrated a bipotent transcriptomic profile, which may contribute to the improvement of their hematopoiesis-supportive function.
体外分析了在 20%（标准实验室）和 5%（组织相关）O 水平下，生长停滞的多能间充质基质细胞（MSCs）中与成骨和成脂分化相关的转录组谱。与第 7 天相比，在第 14 天，在 5%O 下，MSCs 中自发地上调成骨（RUNX2、SP7、BGLAP 和 SPP1）和脂肪分化（CEBPA、PPARG 和 ADIPOQ）基因（p < 0.05）。因此，在组织相关 O 下扩增后，MSCs 表现出双潜能的转录组谱，这可能有助于改善其造血支持功能。
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