Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia.
Biosci Rep. 2020 Dec 23;40(12). doi: 10.1042/BSR20201325.
Collagen has been widely shown to promote osteogenesis of bone marrow mesenchymal stromal cells (BM-MSCs). Due to the invasive procedure of obtaining BM-MSCs, MSCs from other tissues have emerged as a promising alternative for regenerative therapy. MSCs originated from different sources, exhibiting different differentiation potentials. Therefore, the applicability of collagen type I (COL), combining with amniotic membrane (AM)-MSCs was examined through proliferation and differentiation assays together with the expression of surface markers and genes associated with stemness and differentiation under basal or induction conditions. No increase in cell growth was observed because AM-MSCs might be directed toward spontaneous osteogenesis. This was evidenced by the calcium deposition and elevated expression of osteogenic genes when AM-MSCs were cultured in collagen plate with basal media. Under the osteogenic condition, reciprocal expression of OCN and CEBPA suggested a shift toward adipogenesis. Surprisingly, adipogenic genes were not elevated upon adipogenic induction, although oil droplets deposition was observed. In conclusion, our findings demonstrated that collagen causes spontaneous osteogenesis in AM-MSCs. However, the presence of exogenous inductors could shift the direction of adipo-osteogenic gene regulatory network modulated by collagen.
胶原蛋白已被广泛证明可促进骨髓间充质基质细胞（BM-MSCs）的成骨作用。由于获取 BM-MSCs 的侵入性程序，来自其他组织的 MSC 已成为再生治疗的有前途的替代方法。源自不同来源的 MSC 表现出不同的分化潜力。因此，通过增殖和分化测定以及与干性和分化相关的表面标志物和基因的表达，研究了 COL 与羊膜（AM）-MSC 结合的适用性，在基础或诱导条件下。由于 AM-MSCs 可能会自发地向成骨方向分化，因此未观察到细胞生长的增加。这可以通过在基础培养基中的胶原板中培养 AM-MSCs 时钙沉积和骨形成基因的高表达来证明。在成骨条件下，OCN 和 CEBPA 的相互表达表明向脂肪生成的转变。令人惊讶的是，尽管观察到油滴沉积，但在诱导成脂时并未升高脂肪生成基因。总之，我们的研究结果表明胶原蛋白在 AM-MSCs 中引起自发的成骨作用。然而，外源性诱导剂的存在可能会改变由胶原蛋白调节的脂肪 - 成骨基因调控网络的方向。
