School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa, Japan.
Anim Sci J. 2020 Jan-Dec;91(1):e13395. doi: 10.1111/asj.13395.
This study aimed to investigate the effects of whey protein hydrolysate (WPH) on the growth and immunity of mouse pups in artificial rearing (AR) system. Mouse pups were reared in the AR system with artificial milk including 5% WPH (AR with WPH) or not (AR without WPH), and the remaining pups were reared by their mother (dam) for 14 days after birth. The body weight change and body weight gain rates in the AR with WPH group were significantly higher than those observed in the AR without WPH group and similar to those in the dam group. Moreover the feed and protein efficiencies in the AR with WPH group were significantly higher than those of the AR without WPH group. In addition, the supplement of WPH in the AR system was shown to significantly elevate the number of CD3 CD8 , B220 CD19 , IA/IE CD11c , and CD11b in the thymocyte and/or splenocyte, and the thymus weight. Furthermore, MALDI-TOF/MS analysis identified the amino acid sequences corresponding to some peptides, and indicated that VRTPEVDDE had the highest relative intensity among the peptides from tested WPH. Therefore, WPH would be required to not only promote growth, but also exert immunomodulatory activities in mouse pups in AR system.
本研究旨在探讨乳清蛋白水解物（WPH）对人工饲养（AR）系统中小鼠仔鼠生长和免疫的影响。将仔鼠在含有 5% WPH 的人工乳（AR 加 WPH）或不含 WPH 的人工乳（AR 不加 WPH）的 AR 系统中饲养，并在出生后 14 天内让其余仔鼠由其母鼠（母鼠）喂养。AR 加 WPH 组的体重变化和体重增长率明显高于 AR 不加 WPH 组，与母鼠组相似。此外，AR 加 WPH 组的饲料和蛋白质效率明显高于 AR 不加 WPH 组。此外，在 AR 系统中添加 WPH 可显著增加胸腺细胞和/或脾细胞中 CD3 CD8 、B220 CD19 、IA/IE CD11c 和 CD11b 的数量，以及胸腺重量。此外，MALDI-TOF/MS 分析鉴定出与一些肽对应的氨基酸序列，并表明在测试的 WPH 中，VRTPEVDDE 的相对强度最高。因此，WPH 不仅需要促进生长，还需要在 AR 系统中的小鼠仔鼠中发挥免疫调节作用。
