乳清蛋白水解物可修复与年龄相关的和东莨菪碱诱导的认知障碍。

Whey Protein Hydrolysate Renovates Age-Related and Scopolamine-Induced Cognitive Impairment.

机构信息

Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Research and Development, Hilmar Cheese Company, Hilmar, CA 95324, USA.

出版信息

Nutrients. 2023 Feb 28;15(5):1228. doi: 10.3390/nu15051228.

DOI:10.3390/nu15051228

PMID:36904228

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10005054/

Abstract

Whey protein and its hydrolysates are ubiquitously applied in the food system. However, their effect on cognitive impairment remains unclear. This study aimed to investigate the potential ability of whey protein hydrolysate (WPH) to ameliorate cognitive degeneration. WPH intervention in Crl:CD1 (ICR, Institute for cancer research) mice and aged C57BL/6J mice in a scopolamine-induced cognitive impairment model for 10 days were evaluated. Behavioral tests indicated that WPH intervention improved the cognitive abilities in ICR and aged C57BL/6J mice ( < 0.05). Scopolamine enhanced the A level in the brain tissue, and the WPH intervention exhibited a similar therapeutic effect to donepezil in ICR mice. A noticeable reduction occurred in serum A level of aged mice treated with WPH. The histopathological study of the hippocampus showed that WPH intervention alleviates neuronal damage. Hippocampus proteomic analysis suggested possible mechanisms of WPH action. The relative abundance of a gut microbe related to Alzheimer's disease, was altered by WPH intervention. This study demonstrated that short-term WPH intake protected against memory impairment induced by scopolamine and aging.

摘要

乳清蛋白及其水解产物广泛应用于食品体系。然而，它们对认知障碍的影响尚不清楚。本研究旨在探讨乳清蛋白水解物（WPH）改善认知退化的潜在能力。在东莨菪碱诱导的认知障碍模型中，用 WPH 干预 Crl:CD1（ICR，癌症研究所）小鼠和老年 C57BL/6J 小鼠 10 天，评估其效果。行为测试表明，WPH 干预改善了 ICR 和老年 C57BL/6J 小鼠的认知能力（<0.05）。东莨菪碱增强了脑组织中的 A 水平，WPH 干预对 ICR 小鼠的多奈哌齐具有相似的治疗作用。WPH 处理的老年小鼠血清 A 水平明显降低。海马组织的组织病理学研究表明，WPH 干预缓解了神经元损伤。海马蛋白质组学分析提示了 WPH 作用的可能机制。与阿尔茨海默病相关的一种肠道微生物的相对丰度被 WPH 干预改变。本研究表明，短期 WPH 摄入可预防东莨菪碱和衰老引起的记忆障碍。