Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, China.
ACS Appl Mater Interfaces. 2020 Jun 24;12(25):27866-27875. doi: 10.1021/acsami.0c00771. Epub 2020 Jun 15.
Oral biofilms, formed by multiple microorganisms and their extracellular polymeric substances, seriously affect people's life. The emergence of the resistance of biofilms to conventional antibiotics and their side effects on the oral cavity have posed a great challenge in the treatment of dental diseases. Recently, antimicrobial peptides have been recognized as promising alternatives to conventional antibiotics due to their broad antibacterial spectrum, high antibacterial activity, and specific mechanism. However, the research of their anti-biofilm behaviors is still in its infancy, and the underlying mechanism remains unclear. In this study, we investigated the anti-biofilm activities of a designed helical peptide (G3) against (), one of the primary causative pathogens of caries. The results indicated that G3 inhibited biofilm formation by interfering with different stages of biofilm development. At the initial stage, G3 inhibited the bacterial adhesion by decreasing the bacterial surface charges, hydrophobicity, membrane integrity, and adhesion-related gene transcription. At the later stage, G3 interacted with extracellular DNA to destabilize the 3D architecture of mature biofilms and thus dispersed them. The high activity of G3 against biofilms, along with its specific modes of action, endows it great application potential in preventing and treating dental plaque diseases.
口腔生物膜由多种微生物及其细胞外聚合物组成，严重影响人们的生活。生物膜对传统抗生素的耐药性及其对口腔的副作用，给口腔疾病的治疗带来了巨大挑战。最近，由于抗菌肽具有广谱抗菌活性、高抗菌活性和特定的作用机制，已被认为是传统抗生素的有前途的替代品。然而，它们抗生物膜行为的研究仍处于起步阶段，其潜在机制尚不清楚。在这项研究中，我们研究了一种设计的螺旋肽（G3）对（）的抗生物膜活性，（）是龋齿主要致病原之一。结果表明，G3 通过干扰生物膜发育的不同阶段来抑制生物膜的形成。在初始阶段，G3 通过降低细菌表面电荷、疏水性、膜完整性和与粘附相关的基因转录来抑制细菌的粘附。在后期，G3 与细胞外 DNA 相互作用，破坏成熟生物膜的 3D 结构，从而使生物膜分散。G3 对生物膜的高活性及其特定的作用模式赋予了它在预防和治疗牙菌斑疾病方面的巨大应用潜力。
