School of Food and Health, Beijing Technology and Business University, Beijing 100024, China.
College of Food Science and Engineering, Bohai University, Jinzhou 121013, China.
Molecules. 2023 Jan 27;28(3):1236. doi: 10.3390/molecules28031236.
Globally, dental caries is one of the most common non-communicable diseases for patients of all ages; () is its principal pathogen. () shows excellent anti-pathogens and immune-regulation functions in the host. The aim of this study is to evaluate the effects of ET-22 on the formation of biofilms. The living bacteria, heat-killed bacteria, and secretions of ET-22 were prepared using the same number of bacteria. In vitro, they were added into artificial-saliva medium, and used to coculture with the . Results showed that the living bacteria and secretions of ET-22 inhibited biofilm-growth, the synthesis of water-soluble polysaccharide and water-insoluble polysaccharide, and virulence-gene-expression levels related to the formation of biofilms. Surprisingly, the heat-killed ET-22, which is a postbiotic, also showed a similar regulation function. Non-targeted metabonomics technology was used to identify multiple potential active-substances in the postbiotics of ET-22 that inhibit the formation of biofilms, including phenyllactic acid, zidovudine monophosphate, and citrulline. In conclusion, live bacteria and its postbiotics of ET-22 all have inhibitory effects on the formation of biofilm. The postbiotics of ET-22 may be a promising biological anticariogenic-agent.
在全球范围内，龋齿是所有年龄段患者最常见的非传染性疾病之一；（）是其主要病原体。（）在宿主中表现出优异的抗病原体和免疫调节功能。本研究旨在评估 ET-22 对生物膜形成的影响。使用相同数量的细菌制备活细菌、热灭活细菌和 ET-22 的分泌物。在体外，将它们添加到人工唾液培养基中，并与 共培养。结果表明，活细菌和 ET-22 的分泌物抑制生物膜生长、水溶性多糖和水不溶性多糖的合成以及与生物膜形成相关的毒力基因表达水平。令人惊讶的是，热灭活的 ET-22，即后生元，也表现出类似的调节功能。非靶向代谢组学技术用于鉴定 ET-22 后生元中抑制生物膜形成的多种潜在活性物质，包括苯乳酸、齐多夫定单磷酸盐和瓜氨酸。总之，活细菌及其 ET-22 的后生元都对生物膜的形成具有抑制作用。ET-22 的后生元可能是一种有前途的生物抗龋剂。
