Department of Cornea, External Disease, and Refractive Surgery, Instituto de Offtalmología "Conde de Valenciana", Mexico City, Mexico.
Cell and Tissue Biology, Research Unit, Instituto de Oftalmología "Conde de Valenciana IAP", Chimalpopoca 14, Col. Obrera, 06800, Mexico City, Mexico.
Exp Eye Res. 2020 Aug;197:108076. doi: 10.1016/j.exer.2020.108076. Epub 2020 May 30.
The aim of this study was to examine the expression of the cytokines and chemokines receptor-3 (CCR3) molecule in endothelial cells and vascular structures in a murine model of corneal neovascularization and in samples of neovascularized human corneas. An immunofluorescence assay using the murine model showed a greater proportion and intensity of CCR3 in the epithelium and corneal subepithelial regions in corneas with neovascularization. In the absence of vascularization, no CCR3 was found. Of the 32 studied tissues, eight were vascularized and 24 were avascular. In the human corneas, vascularized corneas showed positive labeling for CD31 in all the analzedtissues, as well as positive labeling for CCR3. Therefore, all vascularized tissues showed positive coexpression of CCR3 and CD31, whereas none of the avascular corneas showed immunolabeling for either of these receptors. These results suggest that CCR3 could be a possible marker for corneal neovascularization with potential to be a therapeutic target.
本研究旨在探讨趋化因子受体 3（CCR3）在角膜新生血管小鼠模型的血管内皮细胞和血管结构中的表达，以及在新生血管化人角膜样本中的表达。通过免疫荧光分析，在新生血管化的角膜中，上皮和角膜下皮区域的 CCR3 表达比例和强度更高。在没有血管生成的情况下，未发现 CCR3。在研究的 32 个组织中，有 8 个血管化，24 个无血管化。在人角膜中，所有分析的血管化角膜均显示 CD31 阳性标记，同时也显示 CCR3 阳性标记。因此，所有血管化组织均显示 CCR3 和 CD31 的阳性共表达，而无血管化角膜均未显示这两种受体的免疫标记。这些结果表明，CCR3 可能是角膜新生血管化的一个潜在治疗靶点的候选标志物。
