Department of Ophthalmology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
Acta Ophthalmol. 2013 Aug;91(5):e369-78. doi: 10.1111/aos.12070. Epub 2013 Feb 7.
To explore the inhibitory effects of doxycycline on allograft rejection in alkali-burned cornea beds.
The corneas of BALB/c mice were injured using a 1 mol/l NaOH solution. Following the injury, the corneas from C57BL/6 mice were transplanted into the eyes of BALB/c mice after being randomized into three groups: allogeneic corneal transplantation (group A), topical use of doxycycline after allogeneic corneal transplantation (group B) and syngeneic corneal transplantation (group C). Corneal angiogenesis was examined using whole-mount immunofluorescence, and corneal inflammation was evaluated using inflammation index scoring. The immune rejection of the grafts was examined using a slit lamp. In addition, the expression of vascular endothelial growth factor A and interleukin-1β in the transplanted corneas was examined using a real-time polymerase chain reaction, immunohistochemistry and an enzyme-linked immunosorbent assay.
The outgrowth of the corneal blood vessels in the group A mice was faster than that in the group B and group C mice. The inflammation index levels were highest in the group A mice, intermediate in the group B mice and lowest in the group C mice. Vascular endothelial growth factor and the interleukin-1β protein and mRNA levels decreased dramatically in the group B mice compared with the group A mice (all p-values < 0.01). In addition, the mean survival time in the group B mice (27.00 ± 2.00 days) was significantly longer than that in the group A mice (11.67 ± 1.51 days; p < 0.05).
Doxycycline may have had a significant role in preventing corneal angiogenesis and inflammation in alkali-burned corneal beds, which resulted in higher allograft survival rates.
探讨强力霉素对碱烧伤角膜床同种异体排斥反应的抑制作用。
采用 1mol/L NaOH 溶液损伤 BALB/c 小鼠角膜。损伤后，将 C57BL/6 小鼠的角膜随机分为 3 组：同种异体角膜移植（A 组）、同种异体角膜移植后局部应用强力霉素（B 组）和同基因角膜移植（C 组），移植到 BALB/c 小鼠的眼睛中。通过全层免疫荧光法检测角膜血管生成，通过炎症指数评分评估角膜炎症。用裂隙灯检查移植物的免疫排斥反应。此外，通过实时聚合酶链反应、免疫组织化学和酶联免疫吸附试验检测移植角膜中血管内皮生长因子 A 和白细胞介素-1β的表达。
A 组小鼠角膜血管生长较快，B 组和 C 组小鼠较慢。A 组小鼠的炎症指数水平最高，B 组次之，C 组最低。B 组小鼠血管内皮生长因子和白细胞介素-1β蛋白和 mRNA 水平较 A 组小鼠明显降低（均 p 值<0.01）。此外，B 组小鼠的平均存活时间（27.00±2.00 天）明显长于 A 组小鼠（11.67±1.51 天；p<0.05）。
强力霉素可能在预防碱烧伤角膜床角膜血管生成和炎症方面发挥了重要作用，从而提高了同种异体移植物的存活率。
