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BACKGROUND

The aim of the current study was to investigate and track the SARS-CoV-2 in Iranian Coronavirus Disease 2019 (COVID-19) patients using molecular and phylogenetic methods.

METHODS

We enrolled seven confirmed cases of COVID-19 patients for the phylogenetic assessment of the SARS-CoV-2 in Iran. The nsp-2, nsp-12, and S genes were amplified using one-step RT-PCR and sequenced using Sanger sequencing method. Popular bioinformatics software were used for sequences alignment and analysis as well as phylogenetic construction.

RESULTS

The mean age of the patients in the present study was 60.42 ± 9.94 years and 57.1% (4/7) were male. The results indicated high similarity between Iranian and Chinese strains. We could not find any particular polymorphisms in the assessed regions of the three genes. Phylogenetic trees by neighbor-joining and maximum likelihood method of nsp-2, nsp-12, and S genes showed that there are not any differences between Iranian isolates and those of other countries.

CONCLUSION

As a preliminary phylogenetic study in Iranian SARS-CoV-2 isolates, we found that these isolates are closely related to the Chinese and reference sequences. Also, no sensible differences were observed between Iranian isolates and those of other countries. Further investigations are recommended using more comprehensive methods and larger sample sizes.

背景

本研究旨在采用分子和系统发育学方法，对伊朗 2019 年冠状病毒病（COVID-19）患者中的 SARS-CoV-2 进行调查和跟踪。

方法

我们对 7 例 COVID-19 确诊病例进行了系统发育评估，以检测伊朗的 SARS-CoV-2。使用一步 RT-PCR 扩增 nsp-2、nsp-12 和 S 基因，并使用 Sanger 测序法对其进行测序。使用流行的生物信息学软件进行序列比对和分析以及系统发育构建。

结果

本研究中患者的平均年龄为 60.42 ± 9.94 岁，57.1%（4/7）为男性。结果表明，伊朗和中国株之间具有高度相似性。我们未在这三个基因的评估区域中发现任何特定的多态性。nsp-2、nsp-12 和 S 基因的邻接法和最大似然法构建的系统发育树表明，伊朗分离株与其他国家的分离株之间没有差异。