Lee Sanghoo, Lee Mi-Kyeong, Na Hyeongkyun, Ahn Jinwoo, Hong Gayeon, Lee Youngkee, Park Jimyeong, Kim Yejin, Kim Yun-Tae, Kim Chang-Ki, Lim Hwan-Sub, Lee Kyoung-Ryul
Center for Companion Biomarker, Seoul Clinical Laboratories (SCL) Healthcare Inc., Republic of Korea.
Department of Molecular Diagnostics, Seoul Clinical Laboratories, Republic of Korea.
Gene Rep. 2021 Jun;23:101100. doi: 10.1016/j.genrep.2021.101100. Epub 2021 Mar 23.
The spike (S) protein mutations of SARS-CoV-2 are of major concern in terms of viral transmission and pathogenesis. Hence, we developed a PCR-based method to rapidly detect the 6 mutational hotspots (H49Y, G476S, V483A, H519Q, A520S, and D614G) in the S protein and applied this method to analyze the hotspots in the viral isolates from different geographical origins. Here, we identified that there was only the D614G mutation in the viral isolates. As of September 30, 2020, the analysis of 113,381 sequences available from the public repositories revealed that the SARS-CoV-2 variant carrying G614 has become the most prevalent form globally. Our results support recent epidemiological and genomic data demonstrating that the viral infectivity and transmission are enhanced by the S protein D614G mutation.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的刺突（S）蛋白突变在病毒传播和发病机制方面备受关注。因此，我们开发了一种基于聚合酶链反应（PCR）的方法，用于快速检测S蛋白中的6个突变热点（H49Y、G476S、V483A、H519Q、A520S和D614G），并应用该方法分析来自不同地理区域的病毒分离株中的热点。在此，我们发现病毒分离株中仅存在D614G突变。截至2020年9月30日，对公共数据库中113381条可用序列的分析表明，携带G614的SARS-CoV-2变体已成为全球最流行的形式。我们的结果支持了最近的流行病学和基因组数据，表明S蛋白D614G突变增强了病毒的传染性和传播能力。
