Spectroscopy Department, Physics Division, National Research Centre, El Behooth Str, 12622 Dokki, Cairo, Egypt; Physics Department, Faculty of Science, Taif University, P.O. Box 888, 21974 Taif, Saudi Arabia.
Physics Department, Faculty of Science, Taif University, P.O. Box 888, 21974 Taif, Saudi Arabia; Department of Engineering Physics and Instrumentation, National Institute of Applied Sciences and Technology, Carthage University, Tunisia.
Food Chem. 2020 Oct 30;328:127124. doi: 10.1016/j.foodchem.2020.127124. Epub 2020 May 23.
This work demonstrates the challenges for selenium (Se) determination in bean samples using high-resolution continuum source Graphite Furnace Atomic Absorption Spectrometry (HR-CS-GF-AAS) and line source Graphite Furnace Atomic Absorption Spectrometry (LS-GF-AAS). Different chemical modifiers were optimized namely; Ir, Ru, and Pd/Mg nitrates. At selenium contents <5 ng g, it cannot be quantified accurately. Spectral interferences of the molecular bands of PO and NO as well as the iron lines with Se line at 196.026 were demonstrated. The noticeable low energy of the continuum source of the HR-CS-GF-AAS at 196.026 nm gives high values of the limits of detection and quantification. The Se limits of detection were found to be 24, 33, and 29 ng g for Ir, Ru, and Pd/Mg modifiers, respectively. In situ trapping hydride generation LS-GF-AAS gives better limits of detection and it reached 30 pg g with Ir modifier.
本工作展示了使用高分辨率连续光源石墨炉原子吸收光谱法（HR-CS-GF-AAS）和线光源石墨炉原子吸收光谱法（LS-GF-AAS）测定豆样中硒（Se）的挑战。优化了不同的化学修饰剂，即 Ir、Ru 和 Pd/Mg 硝酸盐。在硒含量<5ng g 时，无法准确定量。证明了 PO 和 NO 的分子带以及铁线与 Se 线在 196.026 处的光谱干扰。HR-CS-GF-AAS 在 196.026nm 处的连续光源能量较低，检出限和定量限的值较高。发现 Ir、Ru 和 Pd/Mg 修饰剂的 Se 检出限分别为 24、33 和 29ng g。采用原位捕集氢化物发生 LS-GF-AAS，检出限更低，用 Ir 修饰剂可达 30pg g。
