Gao H Y, Huang B X, Hou J X, Meng H X
Department of Periodontology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China(Gao Hongyu is working on the Department of Periodontology, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China).
Department of Periodontology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China(Huang Baoxin is working on the Department of Oral Implantology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China).
Zhonghua Kou Qiang Yi Xue Za Zhi. 2020 Jun 9;55(6):402-407. doi: 10.3760/cma.j.cn112144-20191219-00456.
To investigate the systemic expression profile of S100A8 and S100A9 in healthy and inflamed periodontal tissues. Experimental periodontitis models were established by ligations around the mandibular second molars of six beagle dogs for 12 weeks (ligation group). The mandibular second molars on the opposite side were kept clean (healthy control group). The expressions of S100A8 and S100A9 in healthy and inflamed periodontal tissues of six beagle dogs were examined by immunohistochemistry. The expressions of S100A8 and S100A9 in primary human gingival fibroblasts (hGF) from 3 subjects and human periodontal ligament cells (hPDLC) from 3 other subjects were detected by immunocytochemistry. After the ligation for 12 weeks, the mean probing depth of ligation group [(3.86±0.14) mm] was significantly higher than that of healthy control group [(2.11±0.28) mm] (0.01). Results of immunohistochemistry analysis indicated that S100A8 and S100A9 could be expressed in gingival epithelial cells and might infiltrated neutrophils in the healthy periodontium. Except for the gingival epithelial cells and neutrophils, both proteins were induced and expressed in gingival fibroblasts, periodontal ligament cells, microvascular endothelial cells and bone marrow fibroblasts under inflammatory conditions. The distribution of S100A8 and S100A9 differed in the healthy oral gingival epithelium (OGE), which becomes consistent in inflamed OGE. Additionally, the expressions of S100A8 and S100A9 were confirmed in primary hGF and hPDLC. Periodontal inflammation might enlarge the expression scope of S100A8 and S100A9 and enrich multiple cells with expressions of S100A8 and S100A9.
为研究S100A8和S100A9在健康和炎症性牙周组织中的全身表达谱。通过对6只比格犬的下颌第二磨牙进行结扎12周建立实验性牙周炎模型（结扎组）。另一侧的下颌第二磨牙保持清洁（健康对照组）。采用免疫组织化学法检测6只比格犬健康和炎症性牙周组织中S100A8和S100A9的表达。采用免疫细胞化学法检测3名受试者的原代人牙龈成纤维细胞（hGF）和另外3名受试者的人牙周膜细胞（hPDLC）中S100A8和S100A9的表达。结扎12周后，结扎组的平均探诊深度[（3.86±0.14）mm]显著高于健康对照组[（2.11±0.28）mm]（P<0.01）。免疫组织化学分析结果表明，S100A8和S100A9可在牙龈上皮细胞中表达，并且在健康牙周组织中可能浸润中性粒细胞。除牙龈上皮细胞和中性粒细胞外，在炎症条件下，这两种蛋白在牙龈成纤维细胞、牙周膜细胞、微血管内皮细胞和骨髓成纤维细胞中均被诱导表达。S100A8和S100A9在健康的口腔牙龈上皮（OGE）中的分布不同，而在炎症性OGE中变得一致。此外，在原代hGF和hPDLC中证实了S100A8和S100A9的表达。牙周炎症可能扩大S100A8和S100A9的表达范围，并使多种细胞富含S100A8和S100A9的表达。
