嘧啶核苷酸是否像嘌呤核苷酸那样调节珠蛋白mRNA的可翻译性？

Do pyrimidine nucleotides regulate translatability of globin mRNA as purine nucleotides do?

Rubin H N, Almendarez E, Halim M N

Int J Biochem. 1988;20(10):1051-9. doi: 10.1016/0020-711x(88)90249-2.

Abstract

When rabbit globin mRNA was incubated with rabbit reticulocyte lysate in the presence of various concentrations of nucleotides, globin synthesis was inhibited or stimulated dependent on dose. 2. Pyrimidine nucleotides inhibited protein synthesis at 0.3 mM, whereas 2 mM of purine nucleotides were required to cause similar inhibition. 3. Adenosine mono- and diphosphate inhibited globin synthesis at a concentration of only 1 mM; however, the sequence is AMP greater than ADP greater than ATP. 4. Translation arrest by these nucleotides was instantaneous. 5. These results suggest that these nucleotides may provide a structural component for maintaining the integrity, the conformation of mRNA or of the messenger ribonucleoprotein (mRNP).

摘要

当兔珠蛋白信使核糖核酸（mRNA）在不同浓度核苷酸存在的情况下与兔网织红细胞裂解物一起温育时，珠蛋白的合成会根据剂量受到抑制或刺激。2. 嘧啶核苷酸在0.3毫摩尔时抑制蛋白质合成，而嘌呤核苷酸则需要2毫摩尔才能产生类似的抑制作用。3. 单磷酸腺苷（AMP）和二磷酸腺苷（ADP）仅在浓度为1毫摩尔时就抑制珠蛋白合成；然而，抑制顺序为AMP大于ADP大于三磷酸腺苷（ATP）。4. 这些核苷酸引起的翻译停滞是瞬间发生的。5. 这些结果表明，这些核苷酸可能为维持信使核糖核酸（mRNA）或信使核糖核蛋白（mRNP）的完整性和构象提供一种结构成分。