Rubin H N, Halim M N
San Diego Institute of Molecular Biology and Structural RNA, California 92121.
Biochem Mol Biol Int. 1993 Oct;31(2):267-78.
We have used synthetic peptide to study a conserved RNA binding motif in eukaryotic poly(A) binding protein (PABP) as well as its functions. We synthesized an 11 amino-acid peptide based on the consensus sequence (GKSKGFGFV), which is found in all the sequenced eukaryotic PABPs. The synthetic peptide was found to be preferentially bound to the poly(A) alone or the poly(A) attached to the 3'-end of mRNA and not the deadenylated mRNA. Additionally, the 11-mer had strong affinity to bind to ATP. In vitro translation of rabbit globin mRNA poly(A) in the presence of the 11-mer resulted in stimulation of globin synthesis by two-fold as compared to translation in the presence of either deadenylated globin mRNA or globin mRNA poly(A) but in the absence of the 11-mer.
我们已使用合成肽来研究真核生物聚腺苷酸结合蛋白（PABP）中一个保守的RNA结合基序及其功能。我们基于在所有已测序的真核生物PABP中都存在的共有序列（GKSKGFGFV）合成了一种11个氨基酸的肽。发现该合成肽优先与单独的聚腺苷酸或附着于mRNA 3'末端的聚腺苷酸结合，而不与去腺苷酸化的mRNA结合。此外，该11聚体对ATP具有很强的结合亲和力。与在去腺苷酸化的珠蛋白mRNA或珠蛋白mRNA聚腺苷酸存在但不存在该11聚体的情况下进行的翻译相比，在11聚体存在的情况下对兔珠蛋白mRNA聚腺苷酸进行体外翻译导致珠蛋白合成增加了两倍。
