NCAPH 对于非小细胞肺癌细胞的增殖、迁移和侵袭是必需的。
NCAPH Is Required for Proliferation, Migration and Invasion of Non-small-cell Lung Cancer Cells.
机构信息
Department of Premedical Sciences, College of Medicine, Chosun University, Gwangju, Republic of Korea.
Department of Neurosurgery, College of Medicine, Chosun University, Gwangju, Republic of Korea.
出版信息
Anticancer Res. 2020 Jun;40(6):3239-3246. doi: 10.21873/anticanres.14305.
BACKGROUND/AIM: Non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) is implicated in correct chromosome condensation and segregation during mitosis. However, the functional role of NCAPH in the pathogenesis of non-small-cell lung cancer (NSCLC) remains unclear. The aim of this study was to elucidate the role of NCAPH in NSCLC cells.
MATERIALS AND METHODS
A549 and H1299 NSCLC cells were transfected with small-interfering RNA (siRNA) against NCAPH. Subsequently, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony-formation assay and flow cytometry analysis were performed to reveal the role of NCAPH in NSCLC cells. In addition, migration and invasion assay were also performed.
RESULTS
NCAPH knockdown inhibited cell proliferation, induced cell-cycle arrest at G/M phase, and prevented colony formation, migration and invasion by NSCLC cells.
CONCLUSION
NCAPH is involved in NSCLC progression and development, and may be a potential therapeutic target for NSCLC treatment.
背景/目的：非结构性染色体维持凝缩酶复合物亚单位 H（NCAPH）在有丝分裂过程中参与正确的染色体凝聚和分离。然而，NCAPH 在非小细胞肺癌（NSCLC）发病机制中的功能作用尚不清楚。本研究旨在阐明 NCAPH 在 NSCLC 细胞中的作用。
材料和方法
用针对 NCAPH 的小干扰 RNA（siRNA）转染 A549 和 H1299 NSCLC 细胞。随后，进行 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐测定、集落形成测定和流式细胞术分析，以揭示 NCAPH 在 NSCLC 细胞中的作用。此外，还进行了迁移和侵袭测定。
结果
NCAPH 敲低抑制细胞增殖，诱导 NSCLC 细胞在 G/M 期停滞细胞周期，并阻止集落形成、迁移和侵袭。
结论
NCAPH 参与 NSCLC 的进展和发展，可能是 NSCLC 治疗的潜在治疗靶点。
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