Kyogoku H
Biochemistry Laboratory, Cancer Institute, Hokkaido University School of Medicine, Sapporo.
Hokkaido Igaku Zasshi. 1988 Nov;63(6):841-50.
The present study was undertaken to examine malignant alterations of collagen and elastin in human stomach cancer. Results of the study are as follows: 1. Content of hydroxyproline which in characteristic amino acid in collagen was elevated in stomach cancer tissues of Bormann types I to IV as compared to that of the uninvolved stomach. 2. When hydroxyproline content in stomach cancer of type IV (scirrhous) was compared to that in other types (I to III) of the cancer, the content in scirrhous was significantly elevated compared with that in cancers of other types, in terms of dry weight of whole tissue, number of cancer cells, and of insoluble proteins which are rich in collagen and elastin. However, when hydroxyproline was determined on two histological layers (mucosa plus submucosa layer and muscular plus serosa layer) separated from the involved and uninvolved stomach tissues, no significant difference in hydroxyproline content was observed between the scirrhous and non-scirrhous cancers. These observations may imply that an increased collagen synthesis in the scirrhous occurs in many layers of stomach tissue but is not restricted in a particular layer. 3. Non-reducing cross-link amino acids of collagen, pyridinoline and histidinoalanine, were assayed on the involved and uninvolved tissues. Pyridinoline content was higher in stomach cancers of Bormann types I to IV, while no significant difference of histidinoalanine content was found. These observations suggest that there is an increased cross-linking of collagen in stomach cancer. 4. Elastin concentration in stomach cancer was determined through the assay of desmosine and isodesmosine which are specific cross-link amino acids in elastin. The contents of these amino acids was increased in stomach cancer tissues of types I to IV as compared with that in the uninvolved tissue. 5. A ratio of desmosine plus isodesmosine to hydroxyproline was higher in the involved stomach than was in the uninvolved, suggesting that increased elastinosis exceeds collagenosis in stomach cancer.
本研究旨在检测人类胃癌中胶原蛋白和弹性蛋白的恶性改变。研究结果如下：1. 与未受累胃组织相比，Borrmann I型至IV型胃癌组织中作为胶原蛋白特征性氨基酸的羟脯氨酸含量升高。2. 将IV型（硬癌）胃癌中的羟脯氨酸含量与其他类型（I型至III型）癌症中的羟脯氨酸含量进行比较，就整个组织的干重、癌细胞数量以及富含胶原蛋白和弹性蛋白的不溶性蛋白质而言，硬癌中的含量显著高于其他类型癌症。然而，当在从受累和未受累胃组织分离的两个组织学层（黏膜加黏膜下层和肌肉加浆膜层）上测定羟脯氨酸时，硬癌和非硬癌之间在羟脯氨酸含量上未观察到显著差异。这些观察结果可能意味着硬癌中胶原蛋白合成增加发生在胃组织的许多层中，但不限于特定层。3. 在受累和未受累组织上检测了胶原蛋白的非还原性交联氨基酸吡啶啉和组氨酸丙氨酸。吡啶啉含量在Borrmann I型至IV型胃癌中较高，而组氨酸丙氨酸含量未发现显著差异。这些观察结果表明胃癌中胶原蛋白的交联增加。4. 通过检测弹性蛋白中的特异性交联氨基酸锁链素和异锁链素来测定胃癌中的弹性蛋白浓度。与未受累组织相比，I型至IV型胃癌组织中这些氨基酸的含量增加。5. 受累胃组织中锁链素加异锁链素与羟脯氨酸的比值高于未受累胃组织，表明胃癌中弹性蛋白增多超过胶原蛋白增多。
