Water Research Institute, C.N.R, Via Salaria km 29.300, CP 10, 00015, Monterotondo Stazione (Rome), Italy.
Faculty of Science and Technology, Free University of Bozen/Bolzano, Piazza Università 5, 39100, Bolzano, Italy.
Appl Microbiol Biotechnol. 2020 Aug;104(15):6825-6838. doi: 10.1007/s00253-020-10696-8. Epub 2020 Jun 1.
The anaerobic biodegradation of phenol has been realised in a sequencing batch reactor (SBR) under anaerobic conditions with phenol as sole carbon and energy source and with glucose as co-substrate. A step-change increase of phenol loading (from 100 up to 2000 mg/L of phenol concentration in the feed solution) has been applied during the acclimation phase in order to progressively induce the development of a specialised microbial consortium. This approach, combined with the dynamic sequence of operations characterising SBRs and with the high biomass retention time, led to satisfactory phenol and COD removal efficiencies with values > 70% for the highest phenol input (2000 mg/L) fed as the single carbon and energy source. Analysis of removal efficiencies and biodegradation rates suggested that the use of glucose as co-substrate did not induce a significant improvement in process performance. Kinetic tests have been performed at different initial phenol (400-1000 mg/L) and glucose (1880-0 mg/L) concentrations to kinetically characterise the developed biomass: estimated kinetic constants are suitable for application and no inhibitory effect due to high concentrations of phenol has been observed in all investigated conditions. The microbial community has been characterised at different operating conditions through molecular tools: results confirm the successful adaptation-operation approach of the microbial consortium showing a gradual increase in richness and diversity and the occurrence and selection of a high proportion of phenol-degrading genera at the end of the experimentation. Key Points • Anaerobic phenol removal in the range of 70-99% in a sequencing batch reactor. • Negligible effect of co-substrate on removal efficiencies and biodegradation rates. • No biomass inhibition due to phenol concentration in the range of 400-1000 mg/L. • Increasing phenol loads promoted the culture enrichment of phenol-degrading genera.
在序批式反应器（SBR）中，在厌氧条件下，以苯酚为唯一碳源和能源，并以葡萄糖为共基质，实现了苯酚的厌氧生物降解。在驯化阶段，通过逐步提高苯酚的负荷（进料溶液中苯酚浓度从 100 增加到 2000mg/L），逐步诱导出专门的微生物群落。这种方法结合了 SBR 动态操作序列和高生物量停留时间，导致在最高苯酚输入（2000mg/L）下，以单一碳源和能源进料时，苯酚和 COD 的去除效率令人满意，>70%。去除效率和生物降解率的分析表明，使用葡萄糖作为共基质并没有显著提高工艺性能。在不同的初始苯酚（400-1000mg/L）和葡萄糖（1880-0mg/L）浓度下进行了动力学试验，以对开发的生物量进行动力学表征：估计的动力学常数适用于应用，并且在所有研究条件下都没有观察到由于高浓度苯酚而导致的抑制作用。通过分子工具在不同的操作条件下对微生物群落进行了表征：结果证实了微生物群落的成功适应-操作方法，表明在实验结束时，丰富度和多样性逐渐增加，并且出现并选择了高比例的苯酚降解属。关键点 • 在序批式反应器中，苯酚的去除率在 70-99%之间。 • 共基质对去除效率和生物降解率的影响可以忽略不计。 • 在 400-1000mg/L 的苯酚浓度范围内，生物量没有受到抑制。 • 增加苯酚负荷促进了苯酚降解属的培养富集。
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