Department of Otolaryngology Head and Neck Surgery, Universidade Federal de São Paulo/Escola Paulista de Medicina (UNIFESP/EPM), R dos Otonis, 700 - piso superior - Vila Clementino, São Paulo, SP, 04037-004, Brazil.
Department of Otolaryngology, Showa University, Tokyo, Japan.
Eur Arch Otorhinolaryngol. 2021 Feb;278(2):331-338. doi: 10.1007/s00405-020-06094-5. Epub 2020 Jun 1.
In this study, we aimed to determine whether or not COM leads to loss of spiral and Scarpa ganglion neurons.
From the human temporal bone (HTB) collection at the University of Minnesota we selected human temporal bones with COM, defined as the presence of clinically intractable tissue abnormalities in the middle ear (cholesteatoma, perforation of the eardrum, granulation tissue, fibrosis, tympanosclerosis, and cholesterol granuloma). We also selected HTBs from donors with no ear diseases as controls. We quantitatively analyzed the number of spiral and Scarpa ganglion cells and compared the results obtained in the control and study groups.
In both COM and control groups we observed a significant negative correlation between age and number of both spiral (R = -0.632; P < 0.001; 95% CI - 0.766 to - 0.434) and Scarpa ganglion (R = - 0.404; P = 0.008; 95% CI - 0.636 to - 0.051) cells. We did not find any significant differences in the number of spiral ganglion cells (in total or per segment) or in the density of Scarpa ganglion cells (in each vestibular nerve or both) in the COM group as compared with controls (P > 0.05).
Our results did not demonstrate significant loss of cochlear or vestibular peripheral ganglion neuron loss in HTBs with COM as compared with controls.
本研究旨在确定 COM 是否会导致螺旋神经节和 Scarpa 神经节神经元的丧失。
我们从明尼苏达大学的颞骨（HTB）标本库中选择了有 COM 的 HTB，COM 被定义为中耳存在临床上难以治疗的组织异常（胆脂瘤、鼓膜穿孔、肉芽组织、纤维化、鼓室硬化和胆固醇肉芽肿）。我们还选择了来自无耳部疾病供体的 HTB 作为对照。我们定量分析了螺旋神经节和 Scarpa 神经节细胞的数量，并比较了对照组和研究组的结果。
在 COM 组和对照组中，我们都观察到年龄与螺旋神经节（R = -0.632；P < 0.001；95%CI -0.766 至 -0.434）和 Scarpa 神经节（R = -0.404；P = 0.008；95%CI -0.636 至 -0.051）细胞数量之间存在显著的负相关。我们没有发现 COM 组与对照组相比，螺旋神经节细胞数量（总数或每段）或 Scarpa 神经节细胞密度（每根前庭神经或两根）有任何显著差异（P > 0.05）。
与对照组相比，我们的结果并未显示 COM 患者 HTB 中存在明显的耳蜗或前庭外周神经节神经元丧失。
