Zhang Cheng-Cai, Zhang Zi-Xuan, Zhang Wen-Jing, Lu Fu-Hua, Li Rui-Lan, Tan Xian-Rui, Xiang Zeng-Xu
College of Horticulture, Nanjing Agricultural University Nanjing 210095, China.
College of Life Science and Technology, Henan Institute of Science and Technology Xinxiang 453003, China.
Zhongguo Zhong Yao Za Zhi. 2020 Apr;45(8):1893-1900. doi: 10.19540/j.cnki.cjcmm.20200205.104.
The study aims at exploring the expression of differential genes and related metabolic pathways in the process of seed dormancy release. The dormant embryo and the dormant released embryo of Paris polyphylla var. chinensis were used as the test materials, a new generation high-throughput sequencing methods to sequence the transcriptome of the samples was used to carry out systematic bioinformatics analysis. We obtained 62 882 650 and 62 263 366 clean reads from the DNA libraries of the samples before and after dormancy breaking. A total of 69 248 differentially expressed genes(DEGs) were obtained, 56 426 up-regulated genes and 12 822 down-regulated genes. There are 138 267 differentially expressed genes in the process of embryo dormancy release, which were annotated by GO function to 58 subclasses of biological processes, molecular functions and cell components. The annotated differentially expressed genes were closely related to metabolic processes, biological regulation, cell component synthesis and enzyme catalytic activity. We found 139 metabolic pathways through pathway analysis of 58 722 differentially expressed genes. Before and after dormancy, DEGs were mainly enriched in carbon metabolism, secondary metabolite biosynthesis and polysaccharide metabolism. Based on the annotation results in KEGG database, we found 16 metabolic pathways related to the dormancy release of P. polyhoylla var. chinensis. A large number of differentially expressed genes were involved in embryo morphogenesis, polysaccharide decomposition and protein synthesis during seed development and dormancy release. It involves the interaction of multiple metabolic pathways and constitutes a complex regulation network for dormancy relief.
本研究旨在探索种子休眠解除过程中差异基因的表达及相关代谢途径。以华重楼的休眠胚和休眠解除后的胚作为试验材料，采用新一代高通量测序方法对样品转录组进行测序，并进行系统的生物信息学分析。从休眠打破前后样品的DNA文库中分别获得62 882 650和62 263 366条clean reads。共获得69 248个差异表达基因（DEGs），其中上调基因56 426个，下调基因12 822个。在胚休眠解除过程中有138 267个差异表达基因，通过GO功能注释到生物过程、分子功能和细胞组分的58个亚类。注释的差异表达基因与代谢过程、生物调控、细胞组分合成及酶催化活性密切相关。通过对58 722个差异表达基因进行通路分析，发现139条代谢途径。休眠前后，差异表达基因主要富集在碳代谢、次生代谢物生物合成和多糖代谢。基于KEGG数据库中的注释结果，发现16条与华重楼休眠解除相关的代谢途径。大量差异表达基因参与种子发育和休眠解除过程中的胚形态发生、多糖分解和蛋白质合成。它涉及多个代谢途径的相互作用，构成了一个复杂的休眠解除调控网络。
