Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing PR China.
PLoS One. 2019 Feb 21;14(2):e0212514. doi: 10.1371/journal.pone.0212514. eCollection 2019.
We previously analyzed the expression of genes associated with Paris polyphylla var. yunnanensis seed maturation and dormancy release; however, we were unable to clarify the relationship between gene expression levels and these processes. To reveal the molecular mechanisms underlying P. polyphylla var. yunnanensis seed dormancy release during a warm stratification, the transcriptomes of dormant and germinating P. polyphylla var. yunnanensis seeds were separately analyzed by RNA sequencing and were also compared with the transcriptomes of stem-leaf and root tissues harvested during the seed maturation stage. The RNA sequencing of five tissues generated 234,331 unigenes, of which 10,137 (4.33%) were differentially expressed among the analyzed tissues. The 6,619 unigenes whose expression varied among mature dormant, sprouted, and germinated seeds included 95 metabolic and 62 signaling genes related to abscisic acid, gibberellin, auxin, brassinosteroid, cytokinin, ethylene, jasmonic acid and salicylic acid. Additionally, 243 differentially expressed genes were annotated as known seed dormancy/germination-related genes. Among these genes, 109 were regulated by hormones or involved in hormone signal transduction. Finally, 310 transcription factor unigenes, including 71 homologs of known seed dormancy/ germination-related genes, were observed to be differentially expressed during a warm stratification. These results confirm that multiple hormones and transcription factors influence P. polyphylla var. yunnanensis seed dormancy release and germination during a warm stratification. This study identified candidate genes (e.g., ABI5) that should be cloned and functionally characterized regarding their effects on the release of P. polyphylla var. yunnanensis seed morphophysiological dormancy.
我们之前分析了与云南重楼种子成熟和解除休眠相关的基因表达；然而，我们无法阐明基因表达水平与这些过程之间的关系。为了揭示云南重楼种子在温暖层积过程中解除休眠的分子机制，我们分别通过 RNA 测序分析了休眠和萌发的云南重楼种子的转录组，并与种子成熟阶段收获的茎叶和根组织的转录组进行了比较。对五个组织的 RNA 测序共生成 234331 个 unigenes，其中 10137 个（4.33%）在分析的组织中差异表达。在成熟休眠、发芽和萌发种子之间变化的 6619 个 unigenes中，包括 95 个与脱落酸、赤霉素、生长素、油菜素内酯、细胞分裂素、乙烯、茉莉酸和水杨酸相关的代谢和 62 个信号基因。此外，243 个差异表达基因被注释为已知的种子休眠/萌发相关基因。在这些基因中，有 109 个受激素调节或参与激素信号转导。最后，观察到 310 个转录因子 unigenes，包括 71 个已知的种子休眠/萌发相关基因的同源物，在温暖层积过程中差异表达。这些结果证实，多种激素和转录因子影响云南重楼种子在温暖层积过程中的休眠解除和萌发。本研究鉴定了候选基因（例如 ABI5），这些基因应被克隆并对其对云南重楼种子形态生理休眠的释放的功能进行特征分析。
