Construction of irf4a Transgenic Zebrafish Using Tol2 System and Its Potential Application.

PURPOSE

Interferon regulatory factor 4 (IRF4) is identified as a transcriptional factor and plays an important role in the immune response in mammals; however, there are few reports about the function of zebrafish IRF4.

METHODS

We first amplified the coding sequence of from the testis of zebrafish. Besides, the fragments of , , , and vector were added for homologous recombination. By sequencing, we can get the recombinant plasmid and it was microinjected into zebrafish embryos. Fluorescence observation was proceeded at days 3 post fertilization; F0 generations expressing green fluorescence in multiple tissues throughout the body were screened as the founder and raised them to sexual maturity. After mating with WT zebrafish to generate F1 offspring, polymerase chain reaction was used to identify whether was integrated into the zebrafish genome.

CONCLUSION

We obtained the systematic overexpressed transgenic zebrafish with green fluorescence labeled in spine, eyes, heart, brain, and other tissues. The transgenic zebrafish will be used as a tool for the role of IRF4a in the immune response to the inflammation preconditioning in the future study.