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富氢盐水通过上调大鼠脑缺血再灌注后胱硫醚β-合酶（CBS）的表达改善海马神经元凋亡。

Hydrogen-rich saline ameliorates hippocampal neuron apoptosis through up-regulating the expression of cystathionine β-synthase (CBS) after cerebral ischemia- reperfusion in rats.

作者信息

Cong Hai-Ming, Gao Qiu-Ping, Song Guo-Qiang, Ye Ying-Xin, Li Xiao-Li, Zhang Lian-Shuang, Wang Xi-Feng

机构信息

Department of Rehabilitation Medicine, Weihai Central Hospital, Wendeng 264400, China.

Department of Emergency, Ji Nan Zhang Qiu District Hospital of Traditional Chinese Medicine, Zhang Qiu 250200, China.

出版信息

Iran J Basic Med Sci. 2020 Apr;23(4):494-499. doi: 10.22038/ijbms.2020.41751.9857.

DOI:10.22038/ijbms.2020.41751.9857
PMID:32489564
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7239416/
Abstract

OBJECTIVES

This study aimed to evaluate the potential role of hydrogen in rats after cerebral ischemic/reperfusion (I/R) injury.

MATERIALS AND METHODS

The experimental samples were composed of sham group, model group of rats that received middle cerebral artery occlusion (MCAO) for 2 hr followed by reperfusion for 24 hr, and the hydrogen saline group treated by hydro¬gen-rich saline (1 ml/kg) after MCAO. Hydrogen sulfide (H2S), S100-βprotein (S100-β), and neuron-specific enolase (NSE) levels were measured; the levels of malondialdehyde (MDA), reactive oxygen species (ROS), and superoxide dismutase (SOD) were detected; the histologic structure and apoptotic cells of hippocampus were observed; the expressions of cystathionine β-synthase (CBS), nuclear factor erythroid 2-related factor 2 (Nrf2), and hemeoxygenase-1 (HO-1) were measured. Statistical analyses were performed using one-way analysis of variance (ANOVA) followed by Fisher's least significant difference (LSD) test.

RESULTS

Our results showed that hydrogen up-regulated H2S levels via promoting the expression of CBS in the hippocampus, and its treatment alleviated oxidative stress via activating the expression of Nrf2 and HO-1, and then cell apoptosis reduced, furthermore, brain function improved by down-regulating the levels of S100-βand NSE.

CONCLUSION

This study showed that hydrogen-rich saline ameliorates cell injury through up-regulating the expression of CBS in the hippocampus after cerebral ischemia reperfusion (I/R) in rats, this provides new experimental evidence for the treatment of stroke with hydrogen saline.

摘要

目的

本研究旨在评估氢气在大鼠脑缺血/再灌注（I/R）损伤后的潜在作用。

材料与方法

实验样本包括假手术组、大脑中动脉闭塞（MCAO）2小时后再灌注24小时的大鼠模型组，以及MCAO后用富氢盐水（1毫升/千克）处理的富氢盐水组。检测硫化氢（H2S）、S100-β蛋白（S100-β）和神经元特异性烯醇化酶（NSE）水平；检测丙二醛（MDA）、活性氧（ROS）和超氧化物歧化酶（SOD）水平；观察海马的组织结构和凋亡细胞；检测胱硫醚β-合酶（CBS）、核因子红细胞2相关因子2（Nrf2）和血红素加氧酶-1（HO-1）的表达。采用单因素方差分析（ANOVA），随后进行Fisher最小显著差（LSD）检验进行统计分析。

结果

我们的结果表明，氢气通过促进海马中CBS的表达上调H2S水平，其治疗通过激活Nrf2和HO-1的表达减轻氧化应激，进而细胞凋亡减少，此外，通过下调S100-β和NSE水平改善脑功能。

结论

本研究表明，富氢盐水通过上调大鼠脑缺血再灌注（I/R）后海马中CBS的表达减轻细胞损伤，这为用富氢盐水治疗中风提供了新的实验证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10ca/7239416/93b6e67c60fe/IJBMS-23-494-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10ca/7239416/93b6e67c60fe/IJBMS-23-494-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10ca/7239416/93b6e67c60fe/IJBMS-23-494-g007.jpg

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