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长链非编码RNA ZFAS1的敲低抑制非小细胞肺癌中的细胞增殖和转移。

Knockdown of LncRNAZFAS1 suppresses cell proliferation and metastasis in non-small cell lung cancer.

作者信息

Zhou Yong, Hu Xiao-Wei, Yang Si-Jia, Yu Zhe

机构信息

Department of Respiratory Medicine, HwaMei Hospital, University Of Chinese Academy Of Sciences, Ningbo, People's Republic of China.

出版信息

Anim Cells Syst (Seoul). 2020 Mar 2;24(2):107-113. doi: 10.1080/19768354.2020.1736623. eCollection 2020.

DOI:10.1080/19768354.2020.1736623
PMID:32489690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7241457/
Abstract

To evaluate the effects of LncRNAZFAS1 on cell proliferation and tumor metastasis in non-small cell lung cancer (NSCLC), we detected the expression level of LncRNAZFAS1 in NSCLC-related tissues and cells. qRT-PCR results revealed that LncRNAZFAS1 in tumor tissues was significantly higher than that in normal lung tissue, especially significantly up-regulated in stage III / IV and in metastatic NSCLC tissues. LncRNAZFAS1 expression was dramatically up-regulated in 4 NSCLC-related cells (A549, SPC-A1, SK-MES-1, and NCI-H1299), with having the highest expression level in A549 cells. Furthermore, we implemented a knockdown of LncRNAZFAS1 in A549 cells, and the results of CCK8 and Transwell assays suggested that knockdown of LncRNAZFAS1 significantly inhibited NSCLC cell proliferation and metastasis. Next, we constructed a tumor xenograft model to evaluate the effect of LncRNAZFAS1 on the NSCLC cell proliferation . The results indicated that knockdown of LncRNAZFAS1 dramatically inhibited A549 cells proliferation and repressed tumor growth. Additionally, knockdown of LncRNAZFAS1 drastically weakened the expressions of MMP2, MMP9 and Bcl-2 proteins, whereas noticeably strengthened the expression of BAX protein. Our results altogether suggest that knockdown of LncRNAZFAS1 has a negative effect on the proliferation and metastasis of NSCLC cell, which implying LncRNAZFAS1 is a potential unfavorable biomarker in patients with NSCLC.

摘要

为了评估长链非编码RNA ZFAS1（LncRNAZFAS1）对非小细胞肺癌（NSCLC）细胞增殖和肿瘤转移的影响，我们检测了NSCLC相关组织和细胞中LncRNAZFAS1的表达水平。qRT-PCR结果显示，肿瘤组织中的LncRNAZFAS1明显高于正常肺组织，尤其是在III/IV期和转移性NSCLC组织中显著上调。LncRNAZFAS1在4种NSCLC相关细胞（A549、SPC-A1、SK-MES-1和NCI-H1299）中表达显著上调，其中在A549细胞中表达水平最高。此外，我们在A549细胞中敲低LncRNAZFAS1，CCK8和Transwell实验结果表明，敲低LncRNAZFAS1显著抑制NSCLC细胞的增殖和转移。接下来，我们构建了肿瘤异种移植模型来评估LncRNAZFAS1对NSCLC细胞增殖的影响。结果表明，敲低LncRNAZFAS1显著抑制A549细胞的增殖并抑制肿瘤生长。此外，敲低LncRNAZFAS1显著减弱MMP2、MMP9和Bcl-2蛋白的表达，而显著增强BAX蛋白的表达。我们的结果共同表明，敲低LncRNAZFAS1对NSCLC细胞的增殖和转移有负面影响，这意味着LncRNAZFAS1是NSCLC患者潜在的不良生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdd/7241457/e83c7bd64e29/TACS_A_1736623_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdd/7241457/37e52ef9f017/TACS_A_1736623_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdd/7241457/8bfce1fa909f/TACS_A_1736623_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdd/7241457/e83c7bd64e29/TACS_A_1736623_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdd/7241457/37e52ef9f017/TACS_A_1736623_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdd/7241457/8bfce1fa909f/TACS_A_1736623_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdd/7241457/e83c7bd64e29/TACS_A_1736623_F0003_OC.jpg

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